Human MMP-2(Matrix Metalloproteinase 2) ELISA Kit
中文名称：人基质金属蛋白酶2(MMP-2)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.78-50 ng/mL
- 灵敏度： 0.47 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人MMP-2抗体包被于酶标板上，实验时样品（或标准品）中的人MMP-2会与包被抗体结合。后依次加入生物素化的抗人MMP-2抗体和辣根过氧化物酶标记的亲和素，抗人MMP-2抗体与结合在包被抗体上的人MMP-2结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，MMP-2浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中MMP-2的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 ng/mL |
| 灵敏度 | 0.47 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人MMP-2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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The Predictive Role of MMP-2, MMP-9, TIMP-1 and TIMP-2 Serum Levels in the Complete Response of the Tumor to Chemotherapy in Breast Cancer Patients
IF: 1.972Journal： JOURNAL OF INVESTIGATIVE SURGERY (2022)Reactivity：HumanSample Type：serum -
UPLC-ESI-QTOF-MS/MS and 1H-NMR identification of alkaloids in potent fraction of Catharanthus roseus leaves inhibits migration and invasion of MDA-MB-231 cells
IF: 1.653 -
Correlation Between Proteolytic Activity and Abdominal Aortic Aneurysm Wall Morphology with Intraluminal Thrombus Volume
IF: 1.607Journal： ANNALS OF VASCULAR SURGERY (2022)PMID：35779804Reactivity：HumanSample Type：Abdominal Aortic Aneurysm (AAA) Wall -
Geometrically encoded SERS nanobarcodes for the logical detection of nasopharyngeal carcinoma-related progression biomarkers
IF: 14.919 -
The Stiffness of Cardiac Fibroblast Substrates Exerts a Regulatory Influence on Collagen Metabolism via α2β1 Integrin, FAK and Src Kinases
IF: 6.600 -
Anti-proliferation and anti-inflammation effects of corilagin in rheumatoid arthritis by downregulating NF-κB and MAPK signaling pathways
IF: 4.360 -
Serum fibrosis markers could aid in the prediction of factor for successful oral antiviral treatment in hepatitis C
IF: 2.568Journal： EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY (2021)PMID：33560684Reactivity：HumanSample Type：Blood -
Clinical value of 2D speckle tracking imaging in evaluating the effect of percutaneous intramyocardial septal radiofrequency ablation in patients with hypertrophic obstructive cardiomyopathy
IF: 0.910 -
Catharanthus roseus L. extract downregulates the expression profile of motility-related genes in highly invasive human breast cancer cell line MDA-MB-231
IF: 0.811 -
MMP2/MMP9-mediated CD100 shedding is crucial for inducing intrahepatic anti-HBV CD8 T cell responses and HBV clearance
IF: 18.946
实验操作视频
