Human MMP-8(Matrix Metalloproteinase 8) ELISA Kit
中文名称：人基质金属蛋白酶8(MMP-8)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人MMP-8抗体包被于酶标板上，实验时样品（或标准品）中的人MMP-8会与包被抗体结合。后依次加入生物素化的抗人MMP-8抗体和辣根过氧化物酶标记的亲和素，抗人MMP-8抗体与结合在包被抗体上的人MMP-8结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，MMP-8浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中MMP-8的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人MMP-8，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Melatonin supports nonsurgical periodontal treatment in patients with Type 2 diabetes mellitus and periodontitis: A randomized clinical trial
IF: 4.300Journal： JOURNAL OF PERIODONTOLOGY (2023)PMID：38055628Reactivity：HumanSample Type：gingivalcrevicular fluid (GCF) -
MMP-9/BDNF ratio predicts more severe COVID-19 outcomes
IF: 3.642Journal： International Journal of Medical Sciences (2022)Reactivity：HumanSample Type：serum -
Different application procedures of Nd:YAG laser as an adjunct to scaling and root planning in smokers with stage III grade C periodontitis: a single-blind, randomized controlled trial
IF: 2.089Journal： IRISH JOURNAL OF MEDICAL SCIENCE (2022)Reactivity：HumanSample Type：gingival crevicular fluid (GCF) -
Assessing salivary matrix metalloproteinase-8 in prostate cancer patients undergoing androgen deprivation therapy
Journal： Clinical and Experimental Dental Research (2022)DOI：10.1002/cre2.624PMID：35769040Reactivity：HumanSample Type：Salivary -
Chronic Pulmonary Aspergillosis: Disease Severity Using Image Analysis and Correlation with Systemic Proinflammation and Predictors of Clinical Outcome
IF: 5.816 -
Identification of key candidate biomarkers for severe influenza infection by integrated bioinformatical analysis and initial clinical validation
IF: 5.310Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021)PMID：33448094Reactivity：HumanSample Type：Plasma -
Association between isotretinoin (Roaccutanne) use and changes in periodontal clinical parameters and MMP-8 and MMP-9 salivary levels
IF: 4.009Journal： Frontiers in Bioscience-Landmark (2021)DOI：10.52586/4933PMID：34340266Reactivity：HumanSample Type：saliva -
Salivary Metalloproteinase-8 and Metalloproteinase-9 Evaluation in Patients Undergoing Fixed Orthodontic Treatment before and after Periodontal Therapy
IF: 3.390Journal： International Journal of Environmental Research and Public Health (2021)PMID：33567492Reactivity：HumanSample Type：Saliva -
The effect of conventional versus electronic cigarette use on treatment outcomes of peri-implant disease
IF: 2.757 -
Effect of injectable platelet-rich fibrin (i-PRF) on the rate of tooth movement:A randomized clinical trial
IF: 2.079Journal： ANGLE ORTHODONTIST (2021)Reactivity：HumanSample Type：gingival crevicular fluid (GCF)
实验操作视频
