Human MMP-9(Matrix Metalloproteinase 9) ELISA Kit
中文名称：人基质金属蛋白酶9(MMP-9)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人MMP-9抗体包被于酶标板上，实验时样品（或标准品）中的人MMP-9会与包被抗体结合。后依次加入生物素化的抗人MMP-9抗体和辣根过氧化物酶标记的亲和素，抗人MMP-9抗体与结合在包被抗体上的人MMP-9结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，MMP-9浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中MMP-9的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人MMP-9，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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The Stiffness of Cardiac Fibroblast Substrates Exerts a Regulatory Influence on Collagen Metabolism via α2β1 Integrin, FAK and Src Kinases
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Effect of levetiracetam in combination with topiramate on immune function, cognitive function, and neuronal nutritional status of children with intractable epilepsy
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Association between isotretinoin (Roaccutanne) use and changes in periodontal clinical parameters and MMP-8 and MMP-9 salivary levels
IF: 4.009Journal： Frontiers in Bioscience-Landmark (2021)DOI：10.52586/4933PMID：34340266Reactivity：HumanSample Type：saliva -
Lutein Treatment Effects on the Redox Status and Metalloproteinase-9 (MMP-9) in Oral Cancer Squamous Cells—Are There Therapeutical Hopes?
IF: 3.623 -
A Phlorotanin, 6,6′-bieckol from Ecklonia cava, Against Photoaging by Inhibiting MMP-1, -3 and -9 Expression on UVB-induced HaCaT Keratinocytes
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Salivary Metalloproteinase-8 and Metalloproteinase-9 Evaluation in Patients Undergoing Fixed Orthodontic Treatment before and after Periodontal Therapy
IF: 3.390Journal： International Journal of Environmental Research and Public Health (2021)PMID：33567492Reactivity：HumanSample Type：Saliva
实验操作视频
