Human MMP-9(Matrix Metalloproteinase 9) ELISA Kit
中文名称：人基质金属蛋白酶9(MMP-9)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人MMP-9抗体包被于酶标板上，实验时样品（或标准品）中的人MMP-9会与包被抗体结合。后依次加入生物素化的抗人MMP-9抗体和辣根过氧化物酶标记的亲和素，抗人MMP-9抗体与结合在包被抗体上的人MMP-9结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，MMP-9浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中MMP-9的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人MMP-9，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Incremental Accuracy of Blood Biomarkers for Predicting Clinical Outcomes After Intracerebral Hemorrhage
IF: 1.787Journal： Journal of Stroke & Cerebrovascular Diseases (2020)PMID：33338706Reactivity：HumanSample Type：serum -
Catharanthus roseus L. extract downregulates the expression profile of motility-related genes in highly invasive human breast cancer cell line MDA-MB-231
IF: 0.811 -
MMP2/MMP9-mediated CD100 shedding is crucial for inducing intrahepatic anti-HBV CD8 T cell responses and HBV clearance
IF: 18.946 -
Ampelopsin E Reduces the Invasiveness of the Triple Negative Breast Cancer Cell Line, MDA-MB-231
IF: 3.060 -
Monitoring of endostatin, TNF-a VEGFs, MMP-9, and cathepsin-L during three months of diosmin treatment in patients with chronic venous disease (CVD)
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Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis
IF: 3.937Journal： CELLULAR SIGNALLING (2017)PMID：28455144Reactivity：HumanSample Type：Cell culture supernatant -
Obesity, Metabolic syndrome, Diabetes
IF: 3.432Journal： CLINICAL CHEMISTRY AND LABORATORY MEDICINE (2017)Reactivity：HumanSample Type：serum -
Expression and Significance of MMPs in Synovial Fluid, Serum and PBMC Culture Supernatant Stimulated by LPS in Osteoarthritis Patients With or Without Diabetes
IF: 1.685Journal： EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES (2017)PMID：29270923Reactivity：HumanSample Type：Serum,Synovia -
Quantitative phosphoproteomic analysis identifies the critical role of JNK1 in neuroinflammation induced by Japanese encephalitis virus
IF: 7.359 -
Hypermethylation of the Keap1 gene inactivates its function, promotes Nrf2 nuclear accumulation, and is involved in arsenite-induced human keratinocyte transformation
IF: 5.736Journal： FREE RADICAL BIOLOGY AND MEDICINE (2015)PMID：26409248Reactivity：HumanSample Type：Cell culture supernatant,Cell lysate
实验操作视频
