Human MT(Melatonin) ELISA Kit
中文名称：人褪黑素(MT)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用人MT抗原包被于酶标板上，实验时样品或标准品中的人MT与包被的人MT竞争生物素标记的抗人MT单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，人MT浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中人MT的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人MT，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Effects of magnesium and potassium supplementation on insomnia and sleep hormones in patients with diabetes mellitus
IF: 3.900Journal： Frontiers in Endocrinology (2024)Reactivity：HumanSample Type：serum -
Lower plasma melatonin levels in non-hypoxic premature newborns associated with neonatal pain
IF: 3.000Journal： EUROPEAN JOURNAL OF PEDIATRICS (2024)Reactivity：HumanSample Type：plasma -
The relationship between neonatal stress in preterm infants and developmental outcomes at the corrected age of 24–30?months
IF: 2.600Journal： Frontiers in Psychology (2024)Reactivity：HumanSample Type：urine samples -
Potential of epicatechin as antioxidant and antiaging in UV-induced BJ cells by regulating COL1A1, FGF-2, GPX-1, and MMP-1 gene, protein levels, and apoptosis
IF: 2.300 -
The impact of skin-to-skin contact upon stress in preterm infants in a neonatal intensive care unit
IF: 2.100Journal： Frontiers in Pediatrics (2024)Reactivity：HumanSample Type：urine -
Melatonin attenuates sepsis-induced acute kidney injury by promoting mitophagy through SIRT3-mediated TFAM deacetylation
IF: 13.300 -
Multiomics analysis of human peripheral blood reveals marked molecular profiling changes caused by one night of sleep deprivation
IF: 9.900 -
Diabetes, obesity, metabolic syndrome
IF: 6.800Journal： CLINICAL CHEMISTRY AND LABORATORY MEDICINE (2023)Reactivity：HumanSample Type：serum -
Preliminary Study on the Effect of a Night Shift on Blood Pressure and Clock Gene Expression
IF: 5.600Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)PMID：37298261Reactivity：HumanSample Type：blood -
Serum Levels of Neuropeptides in Epileptic Encephalopathy With Spike-and-Wave Activation in Sleep
IF: 3.800
Q1:我想测6-羟基硫酸褪黑素(6-SMT)，能用褪黑素(MT)试剂盒吗？
6-羟基硫酸褪黑素(6-SMT)是褪黑素(Melatonin)的一种主要代谢活性产物，在分子结构上比褪黑素多一个羟基。
褪黑素的ELISA试剂盒理论上会对6-SMT也有一定的交叉识别，但不建议用于检测该指标
实验操作视频
