Human MT(Melatonin) ELISA Kit
中文名称：人褪黑素(MT)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用人MT抗原包被于酶标板上，实验时样品或标准品中的人MT与包被的人MT竞争生物素标记的抗人MT单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，人MT浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中人MT的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人MT，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Melatonin enhances osteoblastogenesis of senescent bone marrow stromal cells through NSD2-mediated chromatin remodelling
IF: 8.554Journal： Clinical and Translational Medicine (2022)DOI：10.1002/ctm2.746PMID：35220680Reactivity：HumanSample Type：bone marrow plasma -
Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway
IF: 7.666 -
Sleep duration of lactating mothers and its relationship with feeding pattern, milk macronutrients and related serum factors: A combined longitudinal cohort and cross-sectional study
IF: 6.590 -
Melatonin regulates proliferation, apoptosis and invasion of trophoblasts in preeclampsia by inhibiting endoplasmic reticulum stress
IF: 3.777Journal： AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2022)PMID：35716034Reactivity：HumanSample Type：serum -
Peculiarities of melatonin levels in preterm infants
IF: 2.275 -
Temporal profile of serum melatonin levels in paediatric pineal tumours subjected to surgery: newer insights
IF: 1.532 -
Increased Expression of Colonic Mucosal Melatonin in Patients with Irritable Bowel Syndrome Correlated with Gut Dysbiosis
IF: 7.691Journal： GENOMICS PROTEOMICS & BIOINFORMATICS (2021)PMID：33607299Reactivity：HumanSample Type：Cell culture supernatant(BON-1 cells) -
Peripheral Clock System Abnormalities in Patients With Parkinson’s Disease
IF: 5.750 -
Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway
IF: 5.719 -
Yoga improves mitochondrial health and reduces severity of autoimmune inflammatory arthritis: A randomized controlled trial
IF: 4.160
实验操作视频
