Human NE/ELA2(Neutrophil Elastase/Elastase-2) ELISA Kit
中文名称：人中性粒细胞弹性蛋白酶(NE/ELA2)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.78-50 ng/mL
- 灵敏度： 0.47 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人NE/ELA2抗体包被于酶标板上，实验时样品（或标准品）中的人NE/ELA2会与包被抗体结合。后依次加入生物素化的抗人NE/ELA2抗体和辣根过氧化物酶标记的亲和素，抗人NE/ELA2抗体与结合在包被抗体上的人NE/ELA2结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，NE/ELA2浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中NE/ELA2的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 ng/mL |
| 灵敏度 | 0.47 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人NE/ELA2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Inhibition of sympathetic tone via hypothalamic descending pathway propagates glucocorticoid-induced endothelial impairment and osteonecrosis of the femoral head
IF: 14.300 -
Towards the Use of Lichens as a Source of Bioactive Substances for Topical Applications
IF: 4.200 -
Circulating microvesicles miR139-3p from bronchopulmonary dysplasia aggravates pulmonary vascular simplification by targeting 4E binding protein 1
IF: 3.500Journal： JOURNAL OF GENE MEDICINE (2024)DOI：10.1002/jgm.3675PMID：38388205Reactivity：HumanSample Type：serum -
Lipoxin A4 suppresses neutrophil extracellular traps formation through the FPR2-dependent regulation of METTL3 in ischemic stroke
IF: 3.500Journal： BRAIN RESEARCH BULLETIN (2024)Reactivity：Rat,HumanSample Type：brain tissue -
Evaluation of the Biological Activity of Hydrogel with Cornus mas L. Extract and Its Potential Use in Dermatology and Cosmetology
IF: 4.600 -
PREDICTIVE VALUE OF NEUTROPHIL EXTRACELLULAR TRAP COMPONENTS FOR 28-DAY ALL-CAUSE MORTALITY IN PATIENTS WITH CARDIAC ARREST: A PILOT OBSERVATIONAL STUDY
IF: 3.100 -
Deep transcranial magnetic stimulation in combination with skin thermography in obesity: a window on sympathetic nervous system
IF: 4.087 -
Can the Prognosis of COVID-19 Disease Be Determined by Fecal Markers and Cytokines?
IF: 3.657Journal： JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022)PMID：36179036Reactivity：HumanSample Type：fecal -
Correlation Between Proteolytic Activity and Abdominal Aortic Aneurysm Wall Morphology with Intraluminal Thrombus Volume
IF: 1.607Journal： ANNALS OF VASCULAR SURGERY (2022)PMID：35779804Reactivity：HumanSample Type：Abdominal Aortic Aneurysm (AAA) Wall -
Identification of key candidate biomarkers for severe influenza infection by integrated bioinformatical analysis and initial clinical validation
IF: 5.310Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021)PMID：33448094Reactivity：HumanSample Type：Plasma
实验操作视频
