Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
中文名称：人中性粒细胞明胶酶相关脂质运载蛋白(NGAL)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人NGAL抗体包被于酶标板上，实验时样品（或标准品）中的人NGAL会与包被抗体结合。后依次加入生物素化的抗人NGAL抗体和辣根过氧化物酶标记的亲和素，抗人NGAL抗体与结合在包被抗体上的人NGAL结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，NGAL浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中NGAL的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人NGAL，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Lipocalin-2 promotes CKD vascular calcification by aggravating VSMCs ferroptosis through NCOA4/FTH1-mediated ferritinophagy
IF: 8.100Journal： Cell Death & Disease (2024)Reactivity：HumanSample Type：serum -
Systematic Review and Meta-analysis of Urine NGAL for AKI in Cirrhosis
IF: 5.700Journal： Kidney International Reports (2024)Reactivity：HumanSample Type：Urine -
Unveiling the role of mtDNA in Liver-Kidney Crosstalk: Insights from trichloroethylene hypersensitivity syndrome
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：HumanSample Type：Serum -
Relationship between low molecular weight heparin calcium therapy and prognosis in severe acute kidney injury in sepsis: Mendelian randomized analysis and retrospective study
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：HumanSample Type：plasma -
Predictive value of?miR-582-5p for onset of?sepsis-induced acute kidney injury and its functional role during disease development
IF: 1.500Journal： Central European Journal of Immunology (2024)Reactivity：HumanSample Type：serum -
The contribution of IL-17A-dependent low LCN2 levels to Helicobacter pylori infection: Insights from clinical and experimental studies
IF: 5.600 -
The Utility of Noninvasive Urinary Biomarkers for the Evaluation of Vesicoureteral Reflux in Children
IF: 5.600Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)PMID：38139407Reactivity：HumanSample Type：urine -
Cardiovascular risk factors and kidney function among automobile mechanic and their association with serum heavy metals in Southwest Nigeria: A cross-sectional study
IF: 3.700 -
Neutrophil gelatinase-associated lipocalin (NGAL) and tumor necrosis factor-α (TNF-α) levels in patients with schizophrenia
IF: 3.400 -
Renal Stem Cells, Renal Resistive Index, and Neutrophil Gelatinase Associated Lipocalin Changes After Revascularization in Patients With Renovascular Hypertension and Ischemic Nephropathy
IF: 3.100
实验操作视频
