Human NT-proBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
中文名称:人氨基端前脑钠素(NT-proBNP)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.09 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人NT-proBNP抗体包被于酶标板上,实验时样品(或标准品)中的人NT-proBNP会与包被抗体结合。后依次加入生物素化的抗人NT-proBNP抗体和辣根过氧化物酶标记的亲和素,抗人NT-proBNP抗体与结合在包被抗体上的人NT-proBNP结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,NT-proBNP浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中NT-proBNP的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.09 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人NT-proBNP,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Irisin Predicts Poor Clinical Outcomes in Patients with Heart Failure with Preserved Ejection Fraction and Low Levels of N-Terminal Pro-B-Type Natriuretic Peptide
IF: 4.800 -
Trajectory of Irisin as a Predictor of Kidney-Related Outcomes in Patients with Asymptomatic Heart Failure
IF: 3.900 -
Low Levels of Adropin Predict Adverse Clinical Outcomes in Outpatients with Newly Diagnosed Prediabetes after Acute Myocardial Infarction
IF: 3.900 -
Predictors for Irreversibility of Contrast-Induced Acute Kidney Injury in Patients with Obesity After Contrast-Enhanced Computed Tomography Coronary Angiography
IF: 3.400Journal: ADVANCES IN THERAPY (2024)Reactivity:HumanSample Type:serum -
Improving DNA Aptamers Against Heart Failure Protein Biomarker Using Structure-Guided Random Mutations Approaches for Colourimetric Biosensor Development
IF: 3.200Journal: Molecular Systems Design & Engineering (2024)Reactivity:HumanSample Type:plasma -
Adropin Predicts Asymptomatic Heart Failure in Patients with Type 2 Diabetes Mellitus Independent of the Levels of Natriuretic Peptides
IF: 3.000 -
Evaluation of some nonroutine cardiac biomarkers among adults and children with beta-thalassemia major
IF: 1.300 -
Predischarge Peak Atrial Longitudinal Strain and Plasma N-terminal Pro-hormone Brain Natriuretic Peptide as a Predictor of Short-term Rehospitalization and Cardiovascular Mortality in Patients with Acute Heart Failure
IF: 0.700Journal: Journal of Cardiovascular Echography (2024)Reactivity:HumanSample Type:Blood sample -
Investigation of the efficacy of beta-blockers and reninangiotensin-aldosterone system inhibitors in chronic heart failure with preserved ejection fraction
IF: 0.600Journal: TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH (2024)Reactivity:HumanSample Type:serum -
Circulating Cell-Free Nuclear DNA Predicted an Improvement of Systolic Left Ventricular Function in Individuals with Chronic Heart Failure with Reduced Ejection Fraction
IF: 0.500Journal: Cardiogenetics (2024)Reactivity:HumanSample Type:Serum