Human ON(Osteonectin) ELISA Kit
中文名称：人骨粘连蛋白(ON)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Human
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人ON抗体包被于酶标板上，实验时样品（或标准品）中的人ON会与包被抗体结合。后依次加入生物素化的抗人ON抗体和辣根过氧化物酶标记的亲和素，抗人ON抗体与结合在包被抗体上的人ON结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，ON浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中ON的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人ON，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Glucagon-like peptide-1 receptor promotes osteoblast differentiation of dental pulp stem cells and bone formation in a zebrafish scale regeneration model
IF: 3.000Elucidating the Osteogenic Potential of Naringin and Naringin–Cu(II) Complex: a Comprehensive In Vitro and In Vivo Study
IF: 1.600Journal： Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2023)Reactivity：HumanNano-encapsulation of melatonin into polydiacetylene-phospholipid assembly for sustained-release and enhanced bone formation in zebrafish
IF: 5.062Journal： JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2022)Reactivity：MouseRutin-Zn(II) complex promotes bone formation - A concise assessment in human dental pulp stem cells and zebrafish
IF: 5.194Bioactive Zinc(II) complex incorporated PCL/gelatin electrospun nanofiber enhanced bone tissue regeneration
IF: 4.384Journal： EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES (2021)PMID：33607242Reactivity：HumanSample Type：Cell culture supernatant(human MG-63 cells)Kaempferol-zinc(II) complex synthesis and evaluation of bone formation using zebrafish model
IF: 3.647Assessment of human ovarian follicular fluid derived mesenchymal stem cells in chitosan/PCL/Zn scaffold for bone tissue regeneration
IF: 3.647Prophylactic supplementation of sinapic acid ameliorates zoledronic acid induced changes in osteoblast survival and differentiation
Journal： Biocatalysis and Agricultural Biotechnology (2020)Reactivity：HumanZinc chelated morin promotes osteoblast differentiation over its uncomplexed counterpart
IF: 2.883Suboptimal Level of Bone-Forming Cells in Advanced Cirrhosis are Associated with Hepatic Osteodystrophy
Journal： Hepatology Communications (2018)PMID：30202823Reactivity：HumanSample Type：Plasma,Tissue Slice
