Human OPG(Osteoprotegerin) ELISA Kit
中文名称：人骨保护素(OPG)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人OPG抗体包被于酶标板上，实验时样品（或标准品）中的人OPG会与包被抗体结合。后依次加入生物素化的抗人OPG抗体和辣根过氧化物酶标记的亲和素，抗人OPG抗体与结合在包被抗体上的人OPG结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，OPG浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中OPG的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人OPG，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Optimization of a Photobiomodulation Protocol to Improve the Cell Viability, Proliferation and Protein Expression in Osteoblasts and Periodontal Ligament Fibroblasts for Accelerated Orthodontic Treatment
IF: 4.700 -
Influence of Ultrasound Stimulation on the Viability, Proliferation and Protein Expression of Osteoblasts and Periodontal Ligament Fibroblasts
IF: 4.700 -
Association of Peri-Implant Keratinized Mucosa Width and Mucosal Thickness with Early Bone Loss: A Cross-Sectional Study
IF: 3.900Journal： Journal of Clinical Medicine (2024)PMID：38610701Reactivity：HumanSample Type：peri-implant crevicular fluid (PICF) -
Randomized clinical trial on the effect of intermittent vibrational force application during orthodontic treatment with aligners on RANKL and OPG concentrations in crevicular fluid
IF: 7.400Journal： Bioengineering & Translational Medicine (2023)PMID：37206229Reactivity：HumanSample Type：crevicular fluid -
Melatonin supports nonsurgical periodontal treatment in patients with Type 2 diabetes mellitus and periodontitis: A randomized clinical trial
IF: 4.300Journal： JOURNAL OF PERIODONTOLOGY (2023)PMID：38055628Reactivity：HumanSample Type：gingivalcrevicular fluid (GCF) -
Osteoprotegerin genetic polymorphisms and their influence on therapeutic response to ibandronate in postmenopausal osteoporotic females
IF: 3.700 -
The effects of exenatide and insulin glargine treatments on bone turnover markers and bone mineral density in postmenopausal patients with type 2 diabetes mellitus
IF: 1.600 -
Panicum miliaceum L. and Bone Healing Properties in Rat Model of Femur Fracture by Activating Phosphate Stimulating Macrophages via BMP2 and RANK Signaling Pathway
IF: 0.700Journal： Pharmacognosy Magazine (2023)Reactivity：HumanSample Type：Femur Tissue -
Effect of Ibandronate Therapy on Serum Chemerin, Vaspin, Omentin-1 and Osteoprotegerin (OPG) in Postmenopausal Osteoporotic Females
IF: 5.988 -
Resveratrol promotes bone mass in ovariectomized rats and the SIRT1 rs7896005 SNP is associated with bone mass in women during perimenopause and early postmenopause
IF: 3.024
实验操作视频
