Human OXA(Orexin A) ELISA Kit
中文名称:人食欲素A/阿立新A(OXA)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 62.5-4000 pg/mL
- 灵敏度: 37.5 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用人OXA抗原包被于酶标板上,实验时样品或标准品中的人OXA与包被的人OXA竞争生物素标记的抗人OXA单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,人OXA浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中人OXA的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 2 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 62.5-4000 pg/mL |
灵敏度 | 37.5 pg/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人OXA,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Evaluation of Orexin-A Salivary Levels and its Correlation with Attention After Non-invasive Brain Stimulation in Female Volleyball Players
IF: 4.100Journal: Sports Medicine-Open (2024)Reactivity:HumanSample Type:salivary -
A proprietary black cumin oil extract (Nigella sativa) (BlaQmax?) modulates stress-sleep-immunity axis safely: Randomized double-blind placebo-controlled study
IF: 5.000 -
Serum Levels of Neuropeptides in Epileptic Encephalopathy With Spike-and-Wave Activation in Sleep
IF: 3.800 -
Correlation of serum Orexin-A level with cognitive function and serum inflammatory cytokines in epileptic patients
IF: 2.200Journal: American Journal of Translational Research (2023)DOI:PMID:37434836PMID:37434836Reactivity:HumanSample Type:serum -
Circulating levels of ghrelin, galanin, and orexin-A orexigenic neuropeptides in obstructive sleep apnea syndrome
IF: 2.816 -
Decreased serum orexin A levels in drug-naive children with attention deficit and hyperactivity disorder
IF: 2.484