Human PAF(Platelet Activating Factor) ELISA Kit

中文名称：人血小板激活因子(PAF)酶联免疫吸附测定试剂盒

货号：E-EL-H2199
文献（7）
说明书

说明书

别称：

Platelet-Activating Factor

Human PAF(Platelet Activating Factor) ELISA Kit

Price: ¥3200.00 ¥2300.00 ¥13600.00

Size:

96 T 48 T 96 T × 5

反应性： Human
检测范围： 78.13-5000 pg/mL
灵敏度： 46.88 pg/mL

产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗人PAF抗体包被于酶标板上，实验时样品（或标准品）中的人PAF会与包被抗体结合。后依次加入生物素化的抗人PAF抗体和辣根过氧化物酶标记的亲和素，抗人PAF抗体与结合在包被抗体上的人PAF结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，PAF浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中PAF的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Human
检测方法	比色法;ELISA;夹心法
检测范围	78.13-5000 pg/mL
灵敏度	46.88 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的人PAF，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

B-cell-activating factor (BAFF) and platelet-activating factor (PAF) in pregnancies complicated by maternal obesity and diabetes: a preliminary study

IF: 1.800

Journal： Journal of Maternal-Fetal & Neonatal Medicine (2023)

DOI：10.1080/14767058.2023.2272010

PMID：37872771

Reactivity：Human

Sample Type：serum
Patterns of mutations in nine cancer-related genes and PAF development among smoking male patients diagnosed with bladder cancer

Journal： TUMOR BIOLOGY (2023)

DOI：10.3233/TUB-220032

PMID：36806529

Reactivity：Human

Sample Type：Serum
Maternal AA/EPA Ratio and Triglycerides as Potential Biomarkers of Patients at Major Risk for Pharmacological Therapy in Gestational Diabetes

IF: 6.706

Journal： Nutrients (2022)

DOI：10.3390/nu14122502

PMID：35745231

Reactivity：Human

Sample Type：plasmatic
Inflammation-mediated changes in haemostatic variables of pulmonary tuberculosis patients during treatment

IF: 2.973

Journal： TUBERCULOSIS (2022)

DOI：10.1016/j.tube.2022.102285

PMID：36436460

Reactivity：Human

Sample Type：plasma
Methylglyoxal, Glycated Albumin, PAF, and TNF-α: Possible Inflammatory and Metabolic Biomarkers for Management of Gestational Diabetes

IF: 4.546

Journal： Nutrients (2020)

DOI：10.3390/nu12020479

PMID：32074941

Reactivity：Human

Sample Type：serum
Food-specific serum IgG and symptom reduction with a personalized, unrestricted-calorie diet of six weeks in Irritable Bowel Syndrome (IBS)

IF: 3.211

Journal： Nutrition & Metabolism (2020)

DOI：10.1186/s12986-020-00528-x

PMID：33292297

Reactivity：Human

Sample Type：blood
Wheat‐dependent exercise‐induced anaphylaxis in Chinese people: a clinical research on 33 cases with antigenic analysis of wheat proteins

IF: 1.977

Journal： CLINICAL AND EXPERIMENTAL DERMATOLOGY (2020)

DOI：10.1111/ced.14035

PMID：31267575

Reactivity：Human

Sample Type：Serum

Species

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Human

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