Human PDGF-BB(Platelet Derived Growth Factor BB) ELISA Kit
中文名称：人血小板衍生生长因子BB(PDGF-BB)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人PDGF-BB抗体包被于酶标板上，实验时样品（或标准品）中的人PDGF-BB会与包被抗体结合。后依次加入生物素化的抗人PDGF-BB抗体和辣根过氧化物酶标记的亲和素，抗人PDGF-BB抗体与结合在包被抗体上的人PDGF-BB结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，PDGF-BB浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中PDGF-BB的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人PDGF-BB，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Biomimetic Triplet Messenger RNA Formulation in MXene Hydrogel Microneedles for Wound Healing
IF: 13.900 -
Ligand-Selective Targeting of Macrophage Hydrogel Elicits Bone Immune-Stem Cell Endogenous Self-Healing Program to Promote Bone Regeneration
IF: 10.000 -
Self-Assembled Peptide Hydrogels Loaded with Umbilical Cord-Derived Mesenchymal Stem Cells Repairing Injured Endometrium and Restoring Fertility
IF: 10.000 -
Janus adhesive microneedle patch loaded with exosomes for intrauterine adhesion treatment
IF: 7.000 -
The therapeutic effect of sufficient oxygen-rich PRP injection in facial rejuvenation by multiple objective evaluations in 26 cases
IF: 3.400Journal： Regenerative Therapy (2024)Reactivity：HumanSample Type：platelet rich plasma (PRP) -
Long-term stability of frozen platelet-rich plasma under??80?°C storage condition
IF: 3.400Journal： Regenerative Therapy (2024)Reactivity：HumanSample Type：Platelet rich plasma (PRP) -
Prevention of Intrauterine Adhesion with Platelet-Rich Plasma Double-Network Hydrogel
IF: 3.200Journal： Advanced Biology (2024)Reactivity：HumanSample Type：whole blood sample -
Comparison of growth factor levels in injectable platelet-rich fibrin obtained from healthy individuals and patients with chronic periodontitis: a pilot study
IF: 2.600 -
Regulation of macrophage subtype via injectable micro/nano-structured porous microsphere for reprogramming osteoimmune microenvironment
IF: 16.744 -
Influence of periodontitis and diabetes on structure and cytokine content of platelet-rich fibrin
IF: 4.068
实验操作视频
