Human PON1(Paraoxonase 1) ELISA Kit
中文名称：人对氧磷酶1(PON1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人PON1抗体包被于酶标板上，实验时样品（或标准品）中的人PON1会与包被抗体结合。后依次加入生物素化的抗人PON1抗体和辣根过氧化物酶标记的亲和素，抗人PON1抗体与结合在包被抗体上的人PON1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，PON1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中PON1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人PON1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Preliminary Metabolomics Study Suggests Favorable Metabolic Changes in the Plasma of Breast Cancer Patients after Surgery and Adjuvant Treatment
IF: 3.900 -
High-Density Lipoprotein-Associated Paraoxonase-1 (PON-1) and Scavenger Receptor Class B Type 1 (SRB-1) in Coronary Artery Disease: Correlation with Disease Severity
IF: 3.000Journal： Journal of Clinical Medicine (2024)Reactivity：HumanSample Type：Serum -
A comprehensive insight from molecular docking and dynamics with clinical investigation on the impact of direct oral anticoagulants on atheroprotective protein in atrial fibrillation
IF: 2.800Journal： BMC Pharmacology & Toxicology (2024)Reactivity：HumanSample Type：serum -
Association of paraoxonase-1 (Q192R) gene polymorphism with coronary artery spasm during cardiac catheterisation in Egyptians
IF: 2.500Journal： ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2024)Reactivity：HumanSample Type：Serum -
Lecithin-cholesterol acyltransferase and paraoxonase-1 levels in atherosclerotic cardiovascular disease patients in Nigeria
IF: 1.000Journal： African Journal of Laboratory Medicine (2024)Reactivity：HumanSample Type：Plasma -
Relationship of paraoxonase-1 and paraoxonase-3 with routine laboratory tests and oxidative stress in type 2 diabetes mellitus
IF: 0.600Journal： Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2024)Reactivity：HumanSample Type：Serum -
Evaluation of Paraoxonase, Arylesterase, Dipeptidyl Peptidase-IV, Oxidative Stress, and Oxidative DNA Damage in Patients with Obsessive-Compulsive Disorder
IF: 0.500Journal： Neurochemical Journal (2024)Reactivity：HumanSample Type：serum -
Serum Arylesterase, Paraoxonase, and Lactonase Activities and Paraoxonase-1 Concentrations in Morbidly Obese Patients and Their Relationship with Non-Alcoholic Steatohepatitis
IF: 7.000 -
Effect of Low-Dose Radiotherapy on the Circulating Levels of Paraoxonase-1-Related Variables and Markers of Inflammation in Patients with COVID-19 Pneumonia
IF: 7.675 -
Associations between myeloperoxidase and paraoxonase-1 and type 2 diabetes in patients with ischemic heart disease
IF: 2.174
实验操作视频
