Human PPAR-γ(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
中文名称:人过氧化物酶体增殖因子活化受体γ(PPAR-γ)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人PPAR-γ抗体包被于酶标板上,实验时样品(或标准品)中的人PPAR-γ会与包被抗体结合。后依次加入生物素化的抗人PPAR-γ抗体和辣根过氧化物酶标记的亲和素,抗人PPAR-γ抗体与结合在包被抗体上的人PPAR-γ结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,PPAR-γ浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中PPAR-γ的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人PPAR-γ,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
The elastin-derived peptide (VGVAPG) activates autophagy in neuroblastoma (SH-SY5Y) cells via peroxisome proliferator-activated receptor gamma (PPARγ)
IF: 3.500 -
Involvement of peroxisome proliferator-activated receptor gamma (PPARγ) and autophagic pathways in the mechanism of action of the tris(2,3-dibromopropyl) isocyanurate (TDBP-TAZTO or TBC) flame retardant in the lung adenocarcinoma (A549) cells in vitro
IF: 3.300 -
Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) Levels in Adolescent with Bipolar Disorder and Their Relationship with Metabolic Parameters
IF: 2.866 -
4-thiazolidinone-based derivatives rosiglitazone and pioglitazone affect the expression of antioxidant enzymes in different human cell lines
IF: 6.530Journal: BIOMEDICINE & PHARMACOTHERAPY (2021)PMID:34243632Reactivity:HumanSample Type:Cell culture supernatant -
Anticancer properties of 5Z-(4-fluorobenzylidene)-2-(4-hydroxyphenylamino)-thiazol-4-one
IF: 4.011Journal: Scientific Reports (2019)PMID:31337851Reactivity:HumanSample Type:Cell culture supernatant -
Boron-rich diet may regulate blood lipid profile and prevent obesity: A non-drug and self-controlled clinical trial
IF: 2.895Journal: JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2019)PMID:31109611Reactivity:HumanSample Type:Serum,Saliva,Urine