Human SOST(Sclerostin) ELISA Kit
中文名称：人硬骨素(SOST)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 62.5-4000 pg/mL
- 灵敏度： 37.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人SOST抗体包被于酶标板上，实验时样品（或标准品）中的人SOST会与包被抗体结合。后依次加入生物素化的抗人SOST抗体和辣根过氧化物酶标记的亲和素，抗人SOST抗体与结合在包被抗体上的人SOST结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，SOST浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中SOST的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 62.5-4000 pg/mL |
| 灵敏度 | 37.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人SOST，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Colchicine Blocks Abdominal Aortic Aneurysm Development by Maintaining Vascular Smooth Muscle Cell Homeostasis
IF: 9.200Journal： International Journal of Biological Sciences (2024)PMID：38617538Reactivity：HumanSample Type：plasma -
Comparative Analysis of Vascular Calcification Risk Factors in Pre-Hemodialysis and Prevalent Hemodialysis Adult Patients: Insights into Calcification Biomarker Associations and Implications for Intervention Strategies in Chronic Kidney Disease
IF: 3.000 -
Changes in Dickkopf-1, but Not Sclerostin, in Gingival Crevicular Fluid Are Associated with Peroral Statin Treatment in Patients with Periodontitis
IF: 2.600Journal： Medicina-Lithuania (2024)PMID：38541234Reactivity：HumanSample Type：Gingival Crevicular Fluid (GCF) -
The Role of Sclerostin in Rheumatic Diseases: A Review
IF: 3.900 -
Evaluation of GCF IL-17, IL-10, TWEAK, and sclerostin levels after scaling and root planing and adjunctive use of diode laser application in patients with periodontitis
IF: 6.993 -
Comparative Evaluation of Sclerostin Levels in Gingival Crevicular Fluid in the Treatment of Chronic Periodontitis Patients Using Diode Laser as an Adjunct to Scaling and Root Planing: A Clinico-biochemical Study
Journal： Contemporary Clinical Dentistry (2021)PMID：34759685Reactivity：HumanSample Type：gingival crevicular fluid (GCF) -
Mountain Bike Racing Stimulates Osteogenic Bone Signaling and Ingesting Carbohydrate-Protein Compared With Carbohydrate-Only Prevents Acute Recovery Bone Resorption Dominance
IF: 2.973Journal： JOURNAL OF STRENGTH AND CONDITIONING RESEARCH (2020)Reactivity：HumanSample Type：Plasma -
The correlation between sclerostin and bone mineral density in renal transplant recipients
IF: 1.544 -
Sclerostin and osteoprotegerin: new markers of chronic kidney disease mediated mineral and bone disease in children
IF: 1.278Journal： JOURNAL OF PEDIATRIC ENDOCRINOLOGY & METABOLISM (2020)PMID：33068383Reactivity：HumanSample Type：serum -
Significance of acPWV for Survival of Hemodialysis Patients
IF: 1.205
实验操作视频
