Human sST2(Soluble ST2) ELISA Kit
中文名称：人可溶性生长刺激表达基因2蛋白(sST2)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人sST2抗体包被于酶标板上，实验时样品（或标准品）中的人sST2会与包被抗体结合。后依次加入生物素化的抗人sST2抗体和辣根过氧化物酶标记的亲和素，抗人sST2抗体与结合在包被抗体上的人sST2结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，sST2浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中sST2的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人sST2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
CDC42 deficiency leads to endometrial stromal cell senescence in recurrent implantation failure
IF: 6.000 -
IL-33/sST2 signaling pathway in pulmonary thromboembolism: A clinical observational study
IF: 3.700 -
Soluble suppression of tumorigenicity 2 associated with major adverse cardiac events in children with myocarditis
IF: 2.800Journal： Frontiers in Cardiovascular Medicine (2024)Reactivity：HumanSample Type：blood samples -
ST2 and IL-33 polymorphisms and the development of childhood asthma: a prospective birth cohort study in Finnish children
IF: 2.200 -
Does the ST2 Level in Pediatric Heart Failure Patients Correlate with Cardiovascular Events and Mortality?
IF: 2.000 -
miR-1268a Regulates Fatty Acid Metabolism by Targeting CD36 in Angiotensin II-induced Heart Failure
IF: 1.800 -
Soluble suppression of tumorigenicity 2 associated with contrast-induced acute kidney injury in patients with STEMI
IF: 1.800Journal： INTERNATIONAL UROLOGY AND NEPHROLOGY (2024)Reactivity：HumanSample Type：blood tissue -
sST2 levels and 3D speckle tracking as predictors of CAD severity in chronic coronary syndrome
IF: 1.400Journal： Egyptian Heart Journal (2024)Reactivity：HumanSample Type：plasma -
Low serum soluble ST2 level in early childhood is associated with the risk for asthma at 7 years of age
IF: 6.800 -
Soluble suppression of tumorigenicity 2 associated with atrial fibrillation detected after stroke: A retrospective study
IF: 4.000
实验操作视频
