Human TIMP-1(Tissue Inhibitors of Metalloproteinase 1) ELISA Kit
中文名称：人基质金属蛋白酶抑制因子1(TIMP-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人TIMP-1抗体包被于酶标板上，实验时样品（或标准品）中的人TIMP-1会与包被抗体结合。后依次加入生物素化的抗人TIMP-1抗体和辣根过氧化物酶标记的亲和素，抗人TIMP-1抗体与结合在包被抗体上的人TIMP-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TIMP-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TIMP-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人TIMP-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Stiff substrates inhibit collagen accumulation via integrin α2β1, FAK and Src kinases in human atrial fibroblast and myofibroblast cultures derived from patients with aortal stenosis
IF: 7.500 -
Kisspeptin-10 increases collagen content in the myocardium by focal adhesion kinase activity
IF: 4.600 -
m6A-modified miR-143-3p inhibits epithelial mesenchymal transition in bronchial epithelial cells and extracellular matrix production in lung fibroblasts by targeting Smad3
IF: 3.200 -
Inhibition Effect of Physalis angulata Leaf Extract on Viability, Collagen Type I, and Tissue Inhibitor of Metalloproteinase 1 (TIMP-1) but Not Plasminogen Activator Inhibitor-1 (PAI-1) of Keloid Fibroblast Culture
IF: 2.300 -
An evaluation of matrix metalloproteinase-9 (Mmp-9) and tissue inhibitor metalloproteinase-1 (Timp-1) Serum levels and the Mmp-9/Timp-1 Ratio in Covid-19 patients
IF: 1.000 -
Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from Sea buckthorn on Hydrogen Peroxide-Induced Aging Human Skin Fibroblasts
IF: 7.675 -
Preparation and evaluation of photoprotective kenaf seed oil nanocarriers-based cream of tocotrienol-rich fraction
IF: 6.449 -
Short isocapnic hyperoxia affects indices of vascular remodeling and intercellular adhesion molecules in healthy men
IF: 2.904Journal： BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH (2022)Reactivity：HumanSample Type：Plasma -
Efficacy of Proanthocyanidins in Nonsurgical Periodontal Therapy
IF: 2.607Journal： INTERNATIONAL DENTAL JOURNAL (2022)PMID：36167610Reactivity：HumanSample Type：saliva samples -
Relationship of interleukin-23 with matrix metalloproteinase-9, pentraxin-3, sleep quality and prostate size in benign prostatic hyperplasia
IF: 2.532
实验操作视频
