Human TIMP-2(Tissue Inhibitors of Metalloproteinase 2) ELISA Kit
中文名称：人基质金属蛋白酶抑制因子2(TIMP-2)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Human
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人TIMP-2抗体包被于酶标板上，实验时样品（或标准品）中的人TIMP-2会与包被抗体结合。后依次加入生物素化的抗人TIMP-2抗体和辣根过氧化物酶标记的亲和素，抗人TIMP-2抗体与结合在包被抗体上的人TIMP-2结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TIMP-2浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TIMP-2的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人TIMP-2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Stiff substrates inhibit collagen accumulation via integrin α2β1, FAK and Src kinases in human atrial fibroblast and myofibroblast cultures derived from patients with aortal stenosis
IF: 7.500Kisspeptin-10 increases collagen content in the myocardium by focal adhesion kinase activity
IF: 4.600TIMP-2 as a predictive biomarker in 5-Fu-resistant colorectal cancer
IF: 3.600Journal： JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY (2023)PMID：36905423Reactivity：HumanSample Type：serumTIMP-2 regulates 5-Fu resistance via the ERK/MAPK signaling pathway in colorectal cancer
IF: 5.955Urinary NGAL, IGFBP-7, and TIMP-2: novel biomarkers to predict contrast medium-induced acute kidney injury in children
IF: 3.222The Predictive Role of MMP-2, MMP-9, TIMP-1 and TIMP-2 Serum Levels in the Complete Response of the Tumor to Chemotherapy in Breast Cancer Patients
IF: 1.972Journal： JOURNAL OF INVESTIGATIVE SURGERY (2022)Reactivity：HumanSample Type：serumThe Stiffness of Cardiac Fibroblast Substrates Exerts a Regulatory Influence on Collagen Metabolism via α2β1 Integrin, FAK and Src Kinases
IF: 6.600Three kinds of corneal host cells contribute differently to corneal neovascularization
IF: 6.680MT1-MMP evaluation in neointimal hyperplasia in the late follow-up after prosthesis implantation
IF: 1.494Journal： INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (2019)PMID：31058412Reactivity：HumanSample Type：neointima
