Human TSP-1(Thrombospondin-1) ELISA Kit
中文名称：人血小板反应蛋白1(TSP-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 ng/mL
- 灵敏度： 4.69 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人TSP-1抗体包被于酶标板上，实验时样品（或标准品）中的人TSP-1会与包被抗体结合。后依次加入生物素化的抗人TSP-1抗体和辣根过氧化物酶标记的亲和素，抗人TSP-1抗体与结合在包被抗体上的人TSP-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TSP-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TSP-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 ng/mL |
| 灵敏度 | 4.69 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人TSP-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Methylation and transcriptomic expression profiles of HUVEC in the oxygen and glucose deprivation model and its clinical implications in AMI patients
IF: 3.700 -
Relationship of Thrombospondin-1 and Thrombospondin-2 with hematological, biochemical and inflammatory markers in COVID-19 patients
IF: 0.700Journal： Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2023)Reactivity：HumanSample Type：serum -
Circulating Levels of Thrombospondin-1 and Thrombospondin-2 in Patients with Temporal Lobe Epilepsy Before and After Surgery.
IF: 1.003 -
Circulating Levels of Thrombospondin-1 and Thrombospondin-2 in Patients with Common Brain Tumors.
IF: 1.003Journal： Turkish Neurosurgery (2021)Reactivity：HumanSample Type：serum -
Discovery of Potential Serum Protein Biomarkers in Ankylosing Spondylitis Using Tandem Mass Tag-Based Quantitative Proteomics
IF: 4.074 -
TIPE2 is negatively correlated with tissue factor and thrombospondin-1 expression in patients with bronchial asthma
IF: 1.410Journal： Experimental and Therapeutic Medicine (2018)PMID：29545867Reactivity：HumanSample Type：serum
实验操作视频
