Human VEGF-C(Vascular Endothelial Growth Factor C) ELISA Kit
中文名称：人血管内皮细胞生长因子C(VEGF-C)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 109.38-7000 pg/mL
- 灵敏度： 65.63 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人VEGF-C抗体包被于酶标板上，实验时样品（或标准品）中的人VEGF-C会与包被抗体结合。后依次加入生物素化的抗人VEGF-C抗体和辣根过氧化物酶标记的亲和素，抗人VEGF-C抗体与结合在包被抗体上的人VEGF-C结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，VEGF-C浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中VEGF-C的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 109.38-7000 pg/mL |
| 灵敏度 | 65.63 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人VEGF-C，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Evaluation of maternal serum vascular endothelial growth factor C and D levels in intrahepatic cholestasis of pregnancy
IF: 3.800Journal： INTERNATIONAL JOURNAL OF GYNECOLOGY & OBSTETRICS (2023)PMID：37680091Reactivity：HumanSample Type：plasma -
Association between the serum concentrations and mutational status of IL?8, IL?27 and VEGF and the expression levels of the hERG potassium channel gene in patients with colorectal cancer
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Tim-3 promotes cell aggressiveness and paclitaxel resistance through NF-κB/STAT3 signalling pathway in breast cancer cells
IF: 4.135Journal： Chinese Journal of Cancer Research (2020)PMID：33223752Reactivity：HumanSample Type：Cell culture supernatant -
The RNA-binding protein QKI suppresses tumorigenesis of clear cell renal cell carcinoma by regulating the expression of HIF-1α
IF: 3.565Journal： Journal of Cancer (2020)PMID：32047543Reactivity：HumanSample Type：Cell culture supernatant -
Effect of Diosmin Administration in Patients with Chronic Venous Disorders on Selected Factors Affecting Angiogenesis
IF: 3.060
实验操作视频
