Human Zonulin ELISA Kit
中文名称：人连蛋白(Zonulin)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.78-50 ng/mL
- 灵敏度： 0.47 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人Zonulin抗体包被于酶标板上，实验时样品（或标准品）中的人Zonulin会与包被抗体结合。后依次加入生物素化的抗人Zonulin抗体和辣根过氧化物酶标记的亲和素，抗人Zonulin抗体与结合在包被抗体上的人Zonulin结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Zonulin浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中Zonulin的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 ng/mL |
| 灵敏度 | 0.47 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人Zonulin，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Assessment of haptoglobin alleles in autism spectrum disorders
IF: 3.998 -
Serum zonulin and claudin-5 levels in patients with schizophrenia
IF: 3.288Journal： EUROPEAN ARCHIVES OF PSYCHIATRY AND CLINICAL NEUROSCIENCE (2020)PMID：32564127Reactivity：HumanSample Type：serum -
Could there be a role of serum zonulin increase in the development of hypercalcemia in primary hyperparathyroidism
IF: 3.235 -
Serum zonulin and claudin-5 levels in children with attention-deficit/hyperactivity disorder
IF: 2.144Journal： INTERNATIONAL JOURNAL OF PSYCHIATRY IN CLINICAL PRACTICE (2020)PMID：32757874Reactivity：HumanSample Type：Serum -
Oxidative stress and gut-derived lipopolysaccharides in children affected by paediatric autoimmune neuropsychiatric disorders associated with streptococcal infections
IF: 1.909 -
Children with Hashimoto’s Thyroiditis Have Increased Intestinal Permeability: Results of a Pilot Study
IF: 1.803Journal： Journal of Clinical Research in Pediatric Endocrinology (2020)PMID：31990165Reactivity：HumanSample Type：serum -
Serum zonulin and claudin-5 levels in children with obsessive–compulsive disorder
IF: 1.780 -
Association of adverse outcomes of intrahepatic cholestasis of pregnancy with zonulin levels
IF: 0.807Journal： JOURNAL OF OBSTETRICS AND GYNAECOLOGY (2020)PMID：33228404Reactivity：HumanSample Type：serum -
Increased Liver Localization of Lipopolysaccharides in Human and Experimental NAFLD
IF: 14.971 -
Does Nox2 Overactivate in Children with Nonalcoholic Fatty Liver Disease?
IF: 5.828
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