Hydroxyproline (HYP) Colorimetric Assay Kit (Acid Hydrolysis Method)
中文名称:羟脯氨酸(HYP)比色法测试盒(酸水解法)

Price:
- 样本类型: 血清(浆);尿液;动物组织
- 检测仪器及波长: 酶标仪(550-570 nm,最佳检测波长为558 nm)
- 研究领域: 氨基酸与蛋白
检测原理 |
样本经水解处理后生成游离羟脯氨酸,其在氧化剂的作用下所产生的氧化产物与显色剂作用反应呈紫红色,在558 nm处有吸收峰,在一定范围内,其吸光度与浓度呈线性关系。通过测定558 nm处吸光度,可计算出样本中羟脯氨酸含量。
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指标简称 | HYP |
灵敏度 | 0.04 μg/mL |
检测范围 | 0.04-10 μg/mL |
精密度 | 批间差:4.4% | 批内差:1.2% |
样本量 | 100 μL |
检测时间 | 50 min |
检测仪器及波长 | 酶标仪(550-570 nm,最佳检测波长为558 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存3个月。 |
有效期 | 3个月 |
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Q1:试剂盒中提到的高浓度盐酸,可以用其他的试剂替代吗?
不能用其他试剂替代,需要使用浓盐酸。
Q2:浓盐酸可以用其他的酸(不是危险品)替代吗?
不可以。
Q3:小鼠皮肤组织推荐稀释倍数?
小鼠皮肤组织参考稀释倍数为20~30倍左右。该稀释倍数仅供参考,由于生物样本间差异性,建议先选取几个样本做下预实验以确定最佳的稀释倍数。

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