Hydroxyproline (HYP) Colorimetric Assay Kit (Acid Hydrolysis Method)
中文名称:羟脯氨酸(HYP)比色法测试盒(酸水解法)

Price:
Size:
96 T (80 samples)
48 T (32 samples)
- 样本类型: 血清(浆);尿液;动物组织
- 检测仪器及波长: 酶标仪(550-570 nm,最佳检测波长为558 nm)
- 研究领域: 氨基酸与蛋白
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
样本经水解处理后生成游离羟脯氨酸,其在氧化剂的作用下所产生的氧化产物与显色剂作用反应呈紫红色,在558 nm处有吸收峰,在一定范围内,其吸光度与浓度呈线性关系。通过测定558 nm处吸光度,可计算出样本中羟脯氨酸含量。
|
指标简称 | HYP |
灵敏度 | 0.04 μg/mL |
检测范围 | 0.04-10 μg/mL |
精密度 | 批间差:4.4% | 批内差:1.2% |
样本量 | 100 μL |
检测时间 | 50 min |
检测仪器及波长 | 酶标仪(550-570 nm,最佳检测波长为558 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存3个月。 |
有效期 | 3个月 |
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