IP3(Inositol Triphosphate) ELISA Kit
中文名称：3磷酸肌醇(IP3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用IP3抗原包被于酶标板上，实验时样品或标准品中的IP3与包被的IP3竞争生物素标记的抗IP3单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，IP3浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中IP3的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的IP3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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A famsin-glucagon axis mediates glucose homeostasis
IF: 27.700Journal： Cell Metabolism (2024)Reactivity：MouseSample Type：islet tissue -
Connexin 36 Mediated Intercellular Endoplasmic Reticulum Stress Transmission Induces SSTA Resistance in Growth Hormone Pituitary Adenoma
IF: 9.200 -
Discovery of N-Substituted Acetamide Derivatives as Promising P2Y14R Antagonists Using Molecular Hybridization Based on Crystallographic Overlay
IF: 6.800 -
Tropomodulin1 exacerbates inflammatory response in macrophages by negatively regulating LPS-induced TLR4 endocytosis
IF: 6.200Journal： CELLULAR AND MOLECULAR LIFE SCIENCES (2024)Reactivity：Mouse -
Discovery of a potent, Highly selective, and In vivo anti-inflammatory Efficacious, P2Y6R antagonist with a novel quinoline-pyrazole scaffold
IF: 6.000Journal： EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2024)Reactivity：Human -
Piperlongumine, a natural alkaloid from Piper longum L. ameliorates metabolic-associated fatty liver disease by antagonizing the thromboxane A2 receptor
IF: 5.300Journal： BIOCHEMICAL PHARMACOLOGY (2024)Reactivity：MouseSample Type：aorta blood tissue -
Alpha-1 adrenergic signaling drives cardiac regeneration via extracellular matrix remodeling transcriptional program in zebrafish macrophages
IF: 11.800 -
Discovery of Selective P2Y6R Antagonists with High Affinity and In Vivo Efficacy for Inflammatory Disease Therapy
IF: 7.300 -
Aspirin Suppresses Hepatic Glucagon Signaling Through Decreasing Production of Thromboxane A2
IF: 4.800 -
Effects of melatonin on dopaminergic neuron development via IP3-mediated mitochondrial Ca2+ regulation in autism spectrum disorder
IF: 3.100
Q1:E-EL-0059 3磷酸肌醇(IP3)酶联免疫吸附测定试剂盒 检测MCF7细胞裂解液时，有没有什么试剂专门能提取MCF7细胞裂解液？
裂解MCM7细胞时，客户可以直接用预冷0.01 M PBS和胰蛋白酶按说明书处理即可，但要注意保证细胞生长状态并保证细胞数量达到10^6。
实验操作视频
