Low-Density Lipoprotein Cholesterol (LDL-C) Colorimetric Assay Kit (Double Reagents)

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中文名称：低密度脂蛋白胆固醇(LDL-C)比色法测试盒(双试剂直接法)

货号：E-BC-K205-M
文献（62）
说明书

说明书

Low-Density Lipoprotein Cholesterol (LDL-C) Colorimetric Assay Kit (Double Reagents)

Price: ¥380.00

Size:

96 T (92 samples)

样本类型： 血清（浆）；细胞
检测仪器及波长： 酶标仪（530-570 nm，最佳检测波长为546 nm）
研究领域： 代谢疾病;糖脂代谢

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根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮计算

检测原理	HDL、CM 和 VLDL 等脂蛋白（除 LDL）首先在表面活性剂作用下改变结构并解离，所释放出来的微粒化胆固醇分子与胆固醇酶试剂反应，产生的过氧化氢在缺乏偶联剂时被消耗而不显色，此时 LDL 颗粒仍是完整的，再加入含有偶联剂的试剂，它可使 LDL 颗粒解离释放胆固醇，胆固醇在胆固醇酯酶(CE )、胆固醇氧化酶 ( CO ) 催化下，产生过氧化氢，过氧化氢在 4-氨基安替吡啉（4-AA）和酚(T-OOS)存在时，经过氧化物酶（POD）催化，反应生成苯醌亚胺非那腙的红色醌类化合物，因其他脂蛋白的胆固醇分子已除去，色泽深浅与 LDL-C 量呈比例。
指标简称	LDL-C
灵敏度	0.04 mmol/L
检测范围	0.04-12 mmol/L
精密度	批间差：10% \| 批内差：5.5%
检测时间	30 min
检测仪器及波长	酶标仪（530-570 nm，最佳检测波长为546 nm）
保存条件	该产品可在 2-8°C避光条件下保存6个月。
有效期	12个月

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