LXA4(Lipoxin A4) ELISA Kit
中文名称：脂氧素A4(LXA4)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 0.78-50 ng/mL
- 灵敏度： 0.47 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用LXA4抗原包被于酶标板上，实验时样品或标准品中的LXA4与包被的LXA4竞争生物素标记的抗LXA4单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，LXA4浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中LXA4的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.78-50 ng/mL |
| 灵敏度 | 0.47 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的LXA4，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Genetically Engineered Macrophages Co-loaded with CD47 Inhibitors Synergistically Reconstruct Efferocytosis and Improve Cardiac Remodeling Post Myocardial Ischemia Reperfusion Injury
IF: 10.000Journal： Advanced Healthcare Materials (2024)PMID：38198534Reactivity：MouseSample Type：cardiac tissue -
The protective effects of lipoxin A4 on type 2 diabetes mellitus: A Chinese prospective cohort study
IF: 5.200 -
Sirtuin6 and Lipoxin A4 levels are decreased in severe periodontitis
IF: 3.400Journal： Clinical Oral Investigations (2023)PMID：37851128Reactivity：HumanSample Type：Saliva,serum -
Targeted delivery and ROS-responsive release of Resolvin D1 by platelet chimeric liposome ameliorates myocardial ischemia–reperfusion injury
IF: 9.429Journal： JOURNAL OF NANOBIOTECHNOLOGY (2022)PMID：36266658Reactivity：MouseSample Type：cardiac tissue -
LXA4 enhances prostate cancer progression by facilitating M2 macrophage polarization via inhibition of METTL3
IF: 5.714Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2022)PMID：35228032Reactivity：HumanSample Type：tumor tissue,pericarcinous tissue -
Pig lung fibrosis is active in the subacute CdCl2 exposure model and exerts cumulative toxicity through the M1/M2 imbalance
IF: 6.291Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2021)PMID：34509164Reactivity：PigSample Type：lung -
Lipoxin A4 activates ALX/FPR2 to attenuate inflammation in Aspergillus fumigatus keratitis
IF: 4.932Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2021)PMID：34162149Reactivity：MouseSample Type：Tissue homogenate -
Cadmium exposure induces TNF-α-mediated necroptosis via FPR2/TGF-β/NF-κB pathway in swine myocardium
IF: 4.221 -
Salivary levels of specialized pro-resolving lipid mediators as indicators of periodontal health/disease status
IF: 4.164Journal： JOURNAL OF CLINICAL PERIODONTOLOGY (2019)PMID：31339183Reactivity：HumanSample Type：saliva -
Infective Larvae of Brugia malayi Induce Polarization of Host Macrophages that Helps in Immune Evasion
IF: 5.511
实验操作视频
