MDA(Malondialdehyde) ELISA Kit
中文名称：丙二醛(MDA)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 31.25-2000 ng/mL
- 灵敏度： 18.75 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用MDA抗原包被于酶标板上，实验时样品或标准品中的MDA与包被的MDA竞争生物素标记的抗MDA单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，MDA浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中MDA的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 ng/mL |
| 灵敏度 | 18.75 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的MDA，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Increased inflammatory cytokines and oxidative stress enhanced antibody production in breast and prostate cancer patients with COVID-19 related depression
IF: 5.500 -
Curculigoside mitigates dextran sulfate sodium?induced colitis by activation of KEAP1?NRF2 interaction to inhibit oxidative damage and autophagy of intestinal epithelium barrier
IF: 5.4Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2023)PMID：37772380Reactivity：Mouse，HumanSample Type：colonic tissue -
Presenilin2 D439A Mutation Induces Dysfunction of Mitochondrial Fusion/Fission Dynamics and Abnormal Regulation of GTPase Activity
IF: 5.1 -
Neuroprotective and Cardiometabolic Role of Vitamin E: Alleviating Neuroinflammation and Metabolic Disturbance Induced by AlCl3 in Rat Models
IF: 4.7 -
Clinical significance of oxidative stress markers as angioinvasion and metastasis indicators in papillary thyroid cancer
IF: 4.6 -
High-fat stimulation induces atrial neural remodeling by reducing NO production via the CRIF1/eNOS/P21 axi
IF: 4.5 -
Mechanisms of anti-ulcer actions of Prangos pabularia (L.) in ethanol-induced gastric ulcer in rats
IF: 4.1Journal： SAUDI PHARMACEUTICAL JOURNAL (2023)PMID：37965491Reactivity：RatSample Type：gastric tissue -
Characterization of Sesuvium sesuvioides Using High-Performance Liquid Chromatography and Validation of Its In Vivo Anti-hyperthyroidism Effect via Suppressing Oxidative Stress and Inflammatory Markers
IF: 4.1 -
Inhibition of cGAS-STING pathway by stress granules after activation of M2 macrophages by human mesenchymal stem cells against drug induced liver injury
IF: 3.6 -
Chlorogenic Acid Attenuates Doxorubicin-Induced Oxidative Stress and Markers of Apoptosis in Cardiomyocytes via Nrf2/HO-1 and Dityrosine Signaling
IF: 3.508Journal： Journal of Personalized Medicine (2023)PMID：37109035Reactivity：RatSample Type：cardiac tissue
实验操作视频
