MDA(Malondialdehyde) ELISA Kit
中文名称：丙二醛(MDA)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 31.25-2000 ng/mL
- 灵敏度： 18.75 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用MDA抗原包被于酶标板上，实验时样品或标准品中的MDA与包被的MDA竞争生物素标记的抗MDA单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，MDA浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中MDA的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 ng/mL |
| 灵敏度 | 18.75 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的MDA，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Shortened Daily Photoperiod Alleviates Anxiety-like Behaviour by Antioxidant Effect and Changes Serum Fatty Acid Profile in Diabetic Rats
IF: 3.508 -
Structural and molecular changes in the rat myocardium following perfluoroctane sulfonate (PFOS) exposure are mitigated by quercetin via modulating HSP 70 and SERCA 2
IF: 3.2 -
TFP5 attenuates cyclin-dependent kinase 5-mediated islet β-cell damage in diabetes
IF: 3.000 -
Chloroform Fraction of Drymaria cordata Linn (CFDC) Suppresses Estradiol Benzoate- Induced Endometrial Hyperplasia
IF: 2.8Journal： Anti-Cancer Agents in Medicinal Chemistry (2023)Reactivity：RatSample Type：serum -
Protective effects of chronic humanin treatment in mice with diabetic encephalopathy: A focus on oxidative stress, inflammation, and apoptosis
IF: 2.7Journal： BEHAVIOURAL BRAIN RESEARCH (2023)PMID：37467966Reactivity：MouseSample Type：prefrontal cortex,hippocampus -
Protective effect of yacon leaves extract (Smallanthus sonchifolius (Poepp.) H. Rob) through antifibrosis, anti-inflammatory, and antioxidant mechanisms toward diabetic nephropathy
IF: 2.100 -
Nrf2 protects against myocardial ischemia-reperfusion injury in diabetic rats by inhibiting Drp1-mediated mitochondrial fission
IF: 2.1 -
δ-Tocotrienol in Combination with Resveratrol Improves the Cardiometabolic Risk Factors and Biomarkers in Patients with Metabolic Syndrome: A Randomized Controlled Trial
IF: 2.100Journal： Metabolic Syndrome and Related Disorders (2023)Reactivity：HumanSample Type：blood sample -
Klotho activation of Nrf2 inhibits the ferroptosis signaling pathway to ameliorate sepsis-associated acute kidney injury
IF: 2.0Journal： Translational Andrology and Urology (2023)PMID：38196698Reactivity：MouseSample Type：serum -
SNHG3/miR-330-5p/HSD11B1 Alleviates Myocardial Ischemia-reperfusion Injury by Regulating the ERK/p38 Signaling Pathway
IF: 1.6
实验操作视频
