MDA(Malondialdehyde) ELISA Kit
中文名称：丙二醛(MDA)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 31.25-2000 ng/mL
- 灵敏度： 18.75 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用MDA抗原包被于酶标板上，实验时样品或标准品中的MDA与包被的MDA竞争生物素标记的抗MDA单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，MDA浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中MDA的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 ng/mL |
| 灵敏度 | 18.75 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的MDA，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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IF: 2.650Journal： Evidence-based Complementary and Alternative Medicine (2022)PMID：35845569Reactivity：RatSample Type：hepatic tissue -
Pharmacological Evaluation of Mentha piperita Against Urolithiasis: An In Vitro and In Vivo Study
IF: 2.623 -
Correlation of Ionized Magnesium with the Parameters of Oxidative Stress as Potential Biomarkers in Patients with Anxiety and Depression: A Pilot Study
IF: 2.623 -
Dietary diversity, diet quality, and oxidative stress in older adults
IF: 2.525 -
Astaxanthin protects against hearing impairment in diabetic rats
IF: 2.474Journal： Brazilian Journal of Otorhinolaryngology (2022)PMID：35331657Reactivity：RatSample Type：serum,cochlea -
Protective effect by low-intensity downhill running training against muscle damage and oxidative stress after high-intensity downhill running in rats
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Stingless bee propolis, metformin, and their combination alleviate diabetic cardiomyopathy
IF: 1.214Journal： Brazilian Journal of Pharmaceutical Sciences (2022)Reactivity：RatSample Type：Heart tissue -
Vitamin E protected the mouse adrenal gland against immobilization stress.
IF: 0.859Journal： POLISH JOURNAL OF VETERINARY SCIENCES (2022)PMID：36155994Reactivity：MouseSample Type：adrenal tissue -
Protective effects of Labisia pumila against neuropathy in a diabetic rat model
Journal： Journal of Diabetes and Metabolic Disorders (2022)PMID：35673507Reactivity：MouseSample Type：serum -
GSTM1 Modulates Expression of Endothelial Adhesion Molecules in Uremic Milieu
IF: 6.543Journal： Oxidative Medicine and Cellular Longevity (2021)PMID：33574979Reactivity：HumanSample Type：Cell lysate
实验操作视频
