MDA(Malondialdehyde) ELISA Kit
中文名称：丙二醛(MDA)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 31.25-2000 ng/mL
- 灵敏度： 18.75 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用MDA抗原包被于酶标板上，实验时样品或标准品中的MDA与包被的MDA竞争生物素标记的抗MDA单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，MDA浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中MDA的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 ng/mL |
| 灵敏度 | 18.75 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的MDA，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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2,3,5,4'-tetrahydroxystilbence-2-O-β-D-glucoside attenuates hepatic steatosis via IKKβ/NF-κB and Keap1-Nrf2 pathways in larval zebrafish
IF: 4.545Journal： BIOMEDICINE & PHARMACOTHERAPY (2020)PMID：32387861Reactivity：FishSample Type：Tissue homogenate -
Cycloastragenol prevents age-related bone loss: Evidence in d-galactose-treated and aged rats
IF: 4.545 -
Endothelial progenitor cell–derived exosomes ameliorate endothelial dysfunction in a mouse model of diabetes
IF: 4.545 -
Cyclovirobuxine D protects against diabetic cardiomyopathy by activating Nrf2-mediated antioxidant responses
IF: 3.998 -
Reproductive effects of subchronic exposure to acetamiprid in male rats
IF: 3.998Journal： Scientific Reports (2020)PMID：32488017Reactivity：RatSample Type：Plasma,Tissue homogenate -
Ameliorative effect of curcumin on lead-induced hematological and hepatorenal toxicity in a rat model
IF: 3.606Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2020)PMID：32125074Reactivity：RatSample Type：serum -
Vitamin E-Bonded Membranes Do Not Influence Markers of Oxidative Stress in Hemodialysis Patients with Homozygous Glutathione Transferase M1 Gene Deletion
IF: 3.531 -
Mechanistic perspective of protective effects of nilotinib against cisplatin-induced testicular injury in rats: Role of JNK/caspase-3 signaling inhibition
IF: 3.292Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020)PMID：32059174Reactivity：RatSample Type：testicular -
Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways
IF: 3.292Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020)PMID：32339907Reactivity：RatSample Type：Serum -
Nicotine-mediated upregulation of microRNA-141 expression determines adipokine-intervened insulin resistance
IF: 3.292Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020)PMID：33002592Reactivity：RatSample Type：Serum
实验操作视频
