MDA(Malondialdehyde) ELISA Kit
中文名称：丙二醛(MDA)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 31.25-2000 ng/mL
- 灵敏度： 18.75 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用MDA抗原包被于酶标板上，实验时样品或标准品中的MDA与包被的MDA竞争生物素标记的抗MDA单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，MDA浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中MDA的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 ng/mL |
| 灵敏度 | 18.75 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的MDA，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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The Ameliorative Effect of Litsea martabanica (Kurz) Hook. f. Leaf Water Extract on Chlorpyrifos-Induced Toxicity in Rats and Its Antioxidant Potentials
IF: 4.7 -
Costunolide Inhibits Chronic Kidney Disease Development by Attenuating IKKβ/NF-κB Pathway
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Tomatidine Alleviates Intervertebral Disc Degeneration by Activating the Nrf2/HO-1/GPX4 Signaling Pathway
IF: 4.7 -
Humanin Attenuates Metabolic, Toxic, and Traumatic Neuropathic Pain in Mice by Protecting Against Oxidative Stress and Increasing Inflammatory Cytokine
IF: 4.6Journal： NEUROPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum -
AuNPs with Cynara scolymus leaf extracts rescue arsenic-induced neurobehavioral deficits and hippocampal tissue toxicity in Balb/c mice through D1R and D2R activation
IF: 4.3Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2024)PMID：38493879Reactivity：MouseSample Type：hippocampal tissue -
Apigenin Ameliorates H2O2-Induced Oxidative Damage in Melanocytes through Nuclear Factor-E2-Related Factor 2 (Nrf2) and Phosphatidylinositol 3-Kinase (PI3K)/Protein Kinase B (Akt)/Mammalian Target of Rapamycin (mTOR) Pathways and Reducing the Generation of Reactive Oxygen Species (ROS) in Zebrafish
IF: 4.3 -
GDF15 attenuates sepsis-induced myocardial dysfunction by inhibiting cardiomyocytes ferroptosis via the SOCS1/GPX4 signaling pathway
IF: 4.2Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)Reactivity：MouseSample Type：cardiac tissue -
5-Methoxytryptophan Alleviates Lipopolysaccharide-Induced Acute Kidney Injury by Regulating Nrf2-Mediated Mitophagy
IF: 4.2Journal： Journal of Inflammation Research (2024)Reactivity：MouseSample Type：kidney tissue -
Effects of canagliflozin preconditioning on post-resuscitation myocardial function in a diabetic rat model of cardiac arrest and cardiopulmonary resuscitation
IF: 4.2Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)Reactivity：RatSample Type：Myocardial tissue -
Assessment of inter-individual variability in hamstring muscle recovery after a sport-specific sprint training in women and men
IF: 4.0
实验操作视频
