Mini Sample Mouse IL-10 (Interleukin 10) ELISA Kit

中文名称：微量法小鼠白介素10(IL-10)酶联免疫吸附测定试剂盒

货号：E-MSEL-M0031
文献（381）
说明书

说明书

别称：

 CSIF、 If2a、 Il-10、 Interleukin-10、 IL10、 Cytokine synthesis inhibitory factor

Mini Sample Mouse IL-10 (Interleukin 10) ELISA Kit

Price: ¥3080.00 ¥2180.00 ¥13860.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 15.63-1000 pg/mL
灵敏度： 9.13 pg/mL

系列介绍	MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4，能有效解决实验样本体积不够的问题。Elabscience^®自主开发的新技术，旨在帮助您以更高效的方式进行科学研究。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-10浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-10抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-10会与包被抗体结合。后依次加入生物素化的抗小鼠IL-10抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-10抗体与结合在包被抗体上的小鼠IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	15.63-1000 pg/mL
灵敏度	9.13 pg/mL
样本体积	25 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠IL-10，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤

Sustained delivery of NT-3 and curcumin augments microenvironment modulation effects of decellularized spinal cord matrix hydrogel for spinal cord injury repair

IF: 5.6

Journal： Regenerative Biomaterials (2024)

DOI：10.1093/rb/rbae039

Reactivity：Mouse
Calycosin alleviates titanium particle-induced osteolysis by modulating macrophage polarization and subsequent osteogenic differentiation

IF: 5.3

Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2024)

DOI：10.1111/jcmm.18157
KAT3B-mediated succinylation of DERL3 suppresses osteogenic differentiation by promoting M1/M2 macrophage polarization

IF: 5.3

Journal： BIOCHEMICAL PHARMACOLOGY (2024)

DOI：10.1016/j.bcp.2024.116724

Reactivity：Mouse
Se/GSH-enriched Candida utilis ameliorates DSS-induced colitis in mice by preventing oxidative stress and improving immune responses

IF: 5.2

Journal： Food Bioscience (2024)

DOI：10.1016/j.fbio.2024.103656
Cucurbitacin IIa promotes the immunogenic cell death?inducing effect of doxorubicin and modulates immune microenvironment in liver cancer

IF: 5.2

Journal： INTERNATIONAL JOURNAL OF ONCOLOGY (2024)

DOI：10.3892/ijo.2024.5625
Activation of the HMGB1-TLR4 pathway impacts the functionality of bone marrow mesenchymal stem cells and disrupts macrophage polarization in immune thrombocytopenia

IF: 5.1

Journal： BRITISH JOURNAL OF HAEMATOLOGY (2024)

DOI：10.1111/bjh.19709

Reactivity：Mouse
Oral delivery of electrohydrodynamically encapsulated Lactiplantibacillus plantarum CRD7 modulates gut health, antioxidant activity, and cytokines-related inflammation and immunity in mice

IF: 5.1

Journal： Food & Function (2024)

DOI：10.1039/D4FO02732A

Reactivity：Mouse

Sample Type：serum
The Role of LC3-Associated Phagocytosis Inhibits the Inflammatory Response in Aspergillus fumigatus Keratitis

IF: 5.0

Journal： INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2024)

DOI：10.1167/iovs.65.8.4

Reactivity：Mouse

Sample Type：corneas tissue
Pigment Dispersion Contributes to Ocular Immune Privilege in a DBA/2J Mouse Model of Pigmentary Glaucoma

IF: 5.0

Journal： INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2024)

DOI：10.1167/iovs.65.8.51

Reactivity：Mouse

Sample Type：Serum
Exercise-induced β-hydroxybutyrate promotes Treg cell differentiation to ameliorate colitis in mice

IF: 4.8

Journal： FASEB JOURNAL (2024)

DOI：10.1096/fj.202301686RR

Species

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Human

Mouse

Porcine

Rat

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