Mini Sample Mouse IL-10 (Interleukin 10) ELISA Kit

中文名称：微量法小鼠白介素10(IL-10)酶联免疫吸附测定试剂盒

货号：E-MSEL-M0031
文献（381）
说明书

说明书

别称：

 CSIF、 If2a、 Il-10、 Interleukin-10、 IL10、 Cytokine synthesis inhibitory factor

Mini Sample Mouse IL-10 (Interleukin 10) ELISA Kit

Price: ¥3080.00 ¥2180.00 ¥13860.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 15.63-1000 pg/mL
灵敏度： 9.13 pg/mL

系列介绍	MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4，能有效解决实验样本体积不够的问题。Elabscience^®自主开发的新技术，旨在帮助您以更高效的方式进行科学研究。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-10浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-10抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-10会与包被抗体结合。后依次加入生物素化的抗小鼠IL-10抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-10抗体与结合在包被抗体上的小鼠IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	15.63-1000 pg/mL
灵敏度	9.13 pg/mL
样本体积	25 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠IL-10，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤

Puerarin Alleviates Experimental Autoimmune Thyroiditis by Regulating Macrophages

IF: 3.6

Journal： JOURNAL OF IMMUNOLOGY (2024)

DOI：10.4049/jimmunol.2300779

Reactivity：Mouse

Sample Type：serum
Sialic acid-modified doxorubicin liposomes target tumor-related immune cells to relieve multiple inhibitions of CD8+ T cells

IF: 3.6

Journal： JOURNAL OF LIPOSOME RESEARCH (2024)

DOI：10.1080/08982104.2023.2298901

Reactivity：Mouse

Sample Type：B16 cell-Xenograft
Mechanically induced M2 macrophages are involved in bone remodeling of the midpalatal suture during palatal expansion

IF: 3.5

Journal： Progress in Orthodontics (2024)

DOI：10.1186/s40510-024-00529-z

Reactivity：Mouse
Study on the mechanism of Magnesium calcium alloys/mineralized collagen composites mediating macrophage polarization to promote bone repair

IF: 3.4

Journal： Heliyon (2024)

DOI：10.1016/j.heliyon.2024.e30279

Reactivity：Mouse
ALKBH5 alleviates lower extremity arteriosclerosis by regulating ITGB1 demethylation and influencing macrophage polarization

IF: 3.4

Journal： Heliyon (2024)

DOI：10.1016/j.heliyon.2024.e41495

Reactivity：Mouse

Sample Type：serum
Mitigation of H2O2 Induced Reactive Oxygen Species and Proinflammatory Cytokines by Buckwheat (Fagopyrum tataricum) Rutin in Cultured RAW264.7 Cells

IF: 3.4

Journal： Biocatalysis and Agricultural Biotechnology (2024)

DOI：10.1016/j.bcab.2024.103462

Reactivity：Mouse
DHA-enriched phosphatidylserine alleviates bisphenol A-induced liver injury through regulating glycerophospholipid metabolism and the SIRT1-AMPK pathway

IF: 3.4

Journal： Heliyon (2024)

DOI：10.1016/j.heliyon.2024.e34835

Reactivity：Mouse

Sample Type：liver tissue
Epimedium polysaccharides ameliorate ulcerative colitis by inhibiting oxidative stress and regulating autophagy

IF: 3.3

Journal： JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2024)

DOI：10.1002/jsfa.14037

Reactivity：Mouse

Sample Type：colon tissue
Sevoflurane blocks KLF5-mediated transcriptional activation of ITGB2 to inhibit macrophage infiltration in hepatic ischemia/reperfusion injury

IF: 3.2

Journal： JOURNAL OF GENE MEDICINE (2024)

DOI：10.1002/jgm.3692

Reactivity：Mouse

Sample Type：serum
Effects of topical isoxsuprine ointment on imiquimod-induced psoriasiform skin inflammation in mice

IF: 3.1

Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)

DOI：10.1007/s00210-024-03359-2

Reactivity：Mouse

Sample Type：skin tissue

Species

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