Mini Sample Mouse IL-10 (Interleukin 10) ELISA Kit
中文名称:微量法小鼠白介素10(IL-10)酶联免疫吸附测定试剂盒

Price:
- 反应性: Mouse
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.13 pg/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-10浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-10抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-10会与包被抗体结合。后依次加入生物素化的抗小鼠IL-10抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-10抗体与结合在包被抗体上的小鼠IL-10结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-10浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-10的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Mouse |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.13 pg/mL |
样本体积 | 25 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠IL-10,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
操作步骤 | ![]() |
-
Effects of different doses of omega-3 polyunsaturated fatty acids on gut microbiota and immunity
IF: 3.894 -
In vitro and in vivo efficacy of thiacloprid against Echinococcus multilocularis
IF: 3.876 -
The osteoimmunomodulatory effect of nanostructured TiF x /TiO x coating on osteogenesis induction
IF: 3.715 -
Glucosylceramide synthase regulates hepatocyte repair after concanavalin A-induced immune-mediated liver injury
IF: 2.984 -
The construction of recombinant Lactobacillus casei vaccine of PEDV and its immune responses in mice
IF: 2.741 -
Plasma Proteins As Biodosimetric Markers of Low-Dose Radiation in Mice
IF: 2.658 -
Anti-Inflammatory and Immunomodulatory Role of Bone Marrow-Derived MSCs in Mice with Acute Lung Injury
IF: 2.607Journal: JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2021)PMID:33471617Reactivity:MouseSample Type:plasma -
HMGB1 augments cognitive impairment in sepsis-associated encephalopathy by binding to MD-2 and promoting NLRP3-induced neuroinflammation
IF: 2.440 -
Evaluation of Allicin Against Alveolar Echinococcosis In Vitro and in a Mouse Model
IF: 1.440 -
Application of mouse model for evaluation of recombinant LpxC and GmhA as novel antigenic vaccine candidates of Glaesserella parasuis serotype 13
IF: 1.267Journal: JOURNAL OF VETERINARY MEDICAL SCIENCE (2021)PMID:34393140Reactivity:MouseSample Type:serum

暂无文件,请在搜索框输入识别码查询相关文件

名称 | 状态 | 有效期至 | 操作 | |
---|---|---|---|---|
{{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注:请在有效期内下载相关文件,过期后页面内将不提供文件下载及预览!