Mini Sample Mouse IL-6 (Interleukin 6) ELISA Kit

中文名称：微量法小鼠白介素6(IL-6)酶联免疫吸附测定试剂盒

货号：E-MSEL-M0001
文献（1289）
说明书

说明书

别称：

Il-6、Il6

Mini Sample Mouse IL-6 (Interleukin 6) ELISA Kit

Price: ¥3080.00 ¥2180.00 ¥13860.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 31.25-2000 pg/mL
灵敏度： 18.75 pg/mL

系列介绍	MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4，能有效解决实验样本体积不够的问题。Elabscience^®自主开发的新技术，旨在帮助您以更高效的方式进行科学研究。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-6浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-6会与包被抗体结合。后依次加入生物素化的抗小鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-6抗体与结合在包被抗体上的小鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	31.25-2000 pg/mL
灵敏度	18.75 pg/mL
样本体积	25 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠IL-6，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤

Anti-inflammatory effect of a novel synthetic compound 1-((4-fluorophenyl)thio)isoquinoline in RAW264.7 macrophages and a zebrafish model

IF: 3.298

Journal： FISH & SHELLFISH IMMUNOLOGY (2019)

DOI：10.1016/j.fsi.2019.01.030

PMID：30685466

Reactivity：Mouse

Sample Type：Cell culture supernatant
Xiaoyaosan prevents atherosclerotic vulnerable plaque formation through heat shock protein/glucocorticoid receptor axis-mediated mechanism

IF: 3.266

Journal： American Journal of Translational Research (2019)

DOI：PMID:31632527

PMID：31632527

Reactivity：Mouse

Sample Type：serum
Propacin, a coumarinolignoid isolated from durian, inhibits the lipopolysaccharide-induced inflammatory response in macrophages through the MAPK and NF-κB pathways

IF: 3.241

Journal： Food & Function (2019)

DOI：10.1039/C9FO02202C

PMID：31859327

Reactivity：Mouse

Sample Type：Cell culture supernatant
Anti-fatigue activity of purified anthocyanins prepared from purple passion fruit (P. edulis Sim) epicarp in mice

IF: 3.197

Journal： Journal of Functional Foods (2019)

DOI：10.1016/j.jff.2019.103725

Reactivity：Mouse

Sample Type：Tissue homogenate
Circular RNA expression profile of liver tissues in an EtOH-induced mouse model of alcoholic hepatitis

IF: 3.170

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2019)

DOI：10.1016/j.ejphar.2019.172642

PMID：31493407

Reactivity：Mouse

Sample Type：Serum,Tissue homogenate
Pancreastatin inhibitor PSTi8 attenuates hyperinsulinemia induced obesity and inflammation mediated insulin resistance via MAPK/NOX3-JNK pathway

IF: 3.170

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2019)

DOI：10.1016/j.ejphar.2019.172723

PMID：31586632

Reactivity：Mouse

Sample Type：Plasma
MMI-0100 Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice through Targeting MK2 Pathway

IF: 3.060

Journal： MOLECULES (2019)

DOI：10.3390/molecules24152832

PMID：31382637

Reactivity：Mouse

Sample Type：colon
Design, Synthesis and Investigation of the Potential Anti-Inflammatory Activity of 7-O-Amide Hesperetin Derivatives

IF: 3.060

Journal： MOLECULES (2019)

DOI：10.3390/molecules24203663

PMID：31614601

Reactivity：Mouse

Sample Type：Cell culture supernatant
Gastrodin attenuates neuroinflammation in DOI-induce Tourette syndrome in rats

IF: 2.965

Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2019)

DOI：10.1002/jbt.22302

PMID：30790395

Reactivity：Rat

Sample Type：Serum,Tissue homogenate
Sinomenine Attenuates Cartilage Degeneration by Regulating miR-223-3p/NLRP3 Inflammasome Signaling

IF: 2.939

Journal： INFLAMMATION (2019)

DOI：10.1007/s10753-019-00986-3

PMID：30847744

Reactivity：Mouse

Sample Type：Serum,Cell culture supernatant

Species

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All

Human

Mouse

Porcine

Rat

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