Mini Sample Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit

中文名称：微量法小鼠单核细胞趋化蛋白1(MCP-1)酶联免疫吸附测定试剂盒

货号：E-MSEL-M0012
文献（100）
说明书

说明书

别称：

Je、Mcp1、Scya2、Ccl2

Mini Sample Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit

Price: ¥3080.00 ¥2180.00 ¥13860.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 15.63-1000 pg/mL
灵敏度： 9.38 pg/mL

系列介绍	MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4，能有效解决实验样本体积不够的问题。Elabscience^®自主开发的新技术，旨在帮助您以更高效的方式进行科学研究。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中MCP-1浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠MCP-1抗体包被于酶标板上，实验时样品（或标准品）中的小鼠MCP-1会与包被抗体结合。后依次加入生物素化的抗小鼠MCP-1抗体和辣根过氧化物酶标记的亲和素，抗小鼠MCP-1抗体与结合在包被抗体上的小鼠MCP-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，MCP-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中MCP-1的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	15.63-1000 pg/mL
灵敏度	9.38 pg/mL
样本体积	25 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠MCP-1，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤

Effect of Intratracheal Instillation of ZnO Nanoparticles on Acute Lung Inflammation Induced by Lipopolysaccharides in Mice

IF: 3.564

Journal： TOXICOLOGICAL SCIENCES (2019)

DOI：10.1093/toxsci/kfz234

PMID：31804693

Reactivity：Mouse

Sample Type：BALF
Anti-IFN-γ Antibody Promotes Osteoclastogenesis in Human Bone Marrow Monocyte-Derived Macrophages Co-Cultured with Tuberculosis-Activated Th1 Cells

IF: 5.500

Journal： CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2018)

DOI：10.1159/000493455

PMID：30205409

Reactivity：Mouse
Propofol attenuates sepsis-induced acute kidney injury by regulating miR-290-5p/CCL-2 signaling pathway

IF: 1.492

Journal： BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH (2018)

DOI：10.1590/1414-431X20187655

PMID：30328934

Reactivity：Mouse

Sample Type：serum
AMP-activated protein kinase reduces inflammatory responses and cellular senescence in pulmonary emphysema

IF: 5.168

Journal： Oncotarget (2017)

DOI：10.18632/oncotarget.15116

PMID：28186975

Reactivity：Mouse

Sample Type：Bronchoalveolar Lavage Fluid
Wu-tou decoction attenuates neuropathic pain via suppressing spinal astrocytic IL-1R1/TRAF6/JNK signaling

IF: 5.168

Journal： Oncotarget (2017)

DOI：10.18632/oncotarget.21638

PMID：29190962

Reactivity：Mouse

Sample Type：Tissue homogenate
Molecular mechanisms of the analgesic action of Wu-tou Decoction on neuropathic pain in mice revealed using microarray and network analysis

IF: 3.223

Journal： ACTA PHARMACOLOGICA SINICA (2017)

DOI：10.1038/aps.2017.110

PMID：28816231

Reactivity：Mouse

Sample Type：Tissue homogenate
Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet

IF: 2.806

Journal： PLoS One (2017)

DOI：10.1371/journal.pone.0185688

PMID：28961281

Reactivity：Mouse

Sample Type：Serum
Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice

IF: 4.492

Journal： Oxidative Medicine and Cellular Longevity (2016)

DOI：10.1155/2016/2715718

PMID：27413418

Reactivity：Mouse

Sample Type：Plasma
Peroxisome proliferator-activated receptor-γ agonist pioglitazone fails to attenuate renal fibrosis caused by unilateral ureteral obstruction in mice

IF: 0.838

Journal： Journal of Huazhong University of Science and Technology-Medical Sciences (2016)

DOI：10.1007/s11596-016-1539-1

PMID：26838738

Reactivity：Mouse

Sample Type：Tissue homogenate
Dendritic cells enhance UHMWPE wear particle-induced osteoclast differentiation of macrophages

IF: 3.369

Journal： JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A (2015)

DOI：10.1002/jbm.a.35459

PMID：25808788

Reactivity：Mouse

Sample Type：Cell culture supernatant

Species

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All

Human

Monkey

Mouse

Rat

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