Mini Sample Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：微量法小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-MSEL-M0002
文献（1619）
说明书

说明书

别称：

DIF、Tnfa、TNF-a、TNFSF2、Tnlg1f、Tnfsf1a、TNFalpha、TNF-alpha、Tnf、Cachectin、Tumor necrosis factor、Tumor necrosis factor ligand superfamily member 2

Mini Sample Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥3080.00 ¥2180.00 ¥13860.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 7.81-500 pg/mL
灵敏度： 4.69 pg/mL

系列介绍	MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4，能有效解决实验样本体积不够的问题。Elabscience^®自主开发的新技术，旨在帮助您以更高效的方式进行科学研究。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中TNF-α浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	7.81-500 pg/mL
灵敏度	4.69 pg/mL
样本体积	25 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤

Original research: Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis

IF: 12.469

Journal： Journal for ImmunoTherapy of Cancer (2022)

DOI：10.1136/jitc-2021-004381

PMID：35738798

Reactivity：Mouse

Sample Type：B16F10 cell-xenograft
Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes

IF: 12.153

Journal： EXPERIMENTAL AND MOLECULAR MEDICINE (2022)

DOI：10.1038/s12276-022-00751-x

PMID：35354913

Reactivity：Mouse

Sample Type：Serum
Nano-sponge-like liposomes remove cholesterol crystals for antiatherosclerosis

IF: 11.467

Journal： JOURNAL OF CONTROLLED RELEASE (2022)

DOI：10.1016/j.jconrel.2022.07.021

PMID：35870569

Reactivity：Mouse

Sample Type：serum
Switchable ROS Scavenger/Generator for MRI-Guided Anti-Inflammation and Anti-Tumor Therapy with Enhanced Therapeutic Efficacy and Reduced Side Effects

IF: 11.092

Journal： Advanced Healthcare Materials (2022)

DOI：10.1002/adhm.202202043

PMID：36367363

Reactivity：Mouse

Sample Type：serum
STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice

IF: 10.787

Journal： Redox Biology (2022)

DOI：10.1016/j.redox.2022.102367

PMID：35724543

Reactivity：Mouse

Sample Type：serum
Suppression of NADPH oxidase 4 inhibits PM2.5-induced cardiac fibrosis through ROS-P38 MAPK pathway

IF: 10.753

Journal： SCIENCE OF THE TOTAL ENVIRONMENT (2022)

DOI：10.1016/j.scitotenv.2022.155558

PMID：35504386

Reactivity：Mouse

Sample Type：lungs, serum, , hearts
Aerofloral studies and allergenicity of dominant pollen types in Taraba and Bauchi States of Northeastern Nigeria

IF: 10.753

Journal： SCIENCE OF THE TOTAL ENVIRONMENT (2022)

DOI：10.1016/j.scitotenv.2022.157076

PMID：35780899

Reactivity：Mouse

Sample Type：plasma
Aloe polymeric acemannan inhibits the cytokine storm in mouse pneumonia models by modulating macrophage metabolism

IF: 10.723

Journal： CARBOHYDRATE POLYMERS (2022)

DOI：10.1016/j.carbpol.2022.120032

PMID：36184177

Reactivity：Mouse

Sample Type：bronchoalveolar fluid
Baicalein self-microemulsion based on drug–phospholipid complex for the alleviation of cytokine storm

IF: 10.684

Journal： Bioengineering & Translational Medicine (2022)

DOI：10.1002/btm2.10357

PMID：36684101

Reactivity：Mouse

Sample Type：plasma
Bioactive Injectable Hydrogel Dressings for Bacteria-Infected Diabetic Wound Healing: A “Pull–Push” Approach

IF: 10.383

Journal： ACS Applied Materials & Interfaces (2022)

DOI：10.1021/acsami.2c04300

PMID：35649246

Reactivity：Mouse

Sample Type：regenerated wound samples

Species

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Canine

Human

Mouse

Porcine

Rat

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