Mini Sample Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：微量法小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-MSEL-M0002
文献（1562）
说明书

说明书

别称：

DIF、Tnfa、TNF-a、TNFSF2、Tnlg1f、Tnfsf1a、TNFalpha、TNF-alpha、Tnf、Cachectin、Tumor necrosis factor、Tumor necrosis factor ligand superfamily member 2

Mini Sample Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥3080.00 ¥2180.00 ¥13860.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 7.81-500 pg/mL
灵敏度： 4.69 pg/mL

系列介绍	MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4，能有效解决实验样本体积不够的问题。Elabscience^®自主开发的新技术，旨在帮助您以更高效的方式进行科学研究。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中TNF-α浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	7.81-500 pg/mL
灵敏度	4.69 pg/mL
样本体积	25 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤

Atrial Natriuretic Peptide (ANP) Suppress λ-Carrageenan-Induced Acute Paw Edema by Modulating Pro-inflammatory Genes in Swiss Albino Mice

IF: 2.191

Journal： International Journal of Peptide Research and Therapeutics (2022)

DOI：10.1007/s10989-022-10444-8

Reactivity：Mouse

Sample Type：paw tissue
Adipose-Derived Stem Cells-Derived Exosomes with High Amounts of Circ_0001747 Alleviate Hypoxia/Reoxygenation-Induced Injury in Myocardial Cells by Targeting MiR-199b-3p/MCL1 Axis

IF: 1.823

Journal： International Heart Journal (2022)

DOI：10.1536/ihj.21-441

PMID：35354755

Reactivity：Mouse
Acacetin improves cognitive function of APP/PS1 Alzheimer’s disease model mice via the NLRP3 inflammasome signaling pathway

IF: 1.264

Journal： Translational Neuroscience (2022)

DOI：10.1515/tnsci-2022-0254

Reactivity：Mouse

Sample Type：Brain tissue
Antigen Presentation and Proteome Study of Exosomes Secreted by Co-Culture of Macrophages and Talaromyces marneffei.

IF: 1.167

Journal： FOLIA BIOLOGICA (2022)

DOI：PMID:36871168

Reactivity：Mouse
A Novel Hepatectomy Model in Mice Using a Gutta Cutter Tool: A Feasibility Study and Preliminary Results

IF: 1.014

Journal： TRANSPLANTATION PROCEEDINGS (2022)

DOI：10.1016/j.transproceed.2022.01.029

PMID：35287968

Reactivity：Mouse

Sample Type：serum
Anti-inflammatory effect of luteolin-7-O-glucoside via the JAK1/STAT6/SOCS1 pathway in ulcerative colitis treatment

IF: 0.948

Journal： Pharmacognosy Magazine (2022)

DOI：10.4103/pm.pm_506_21

Reactivity：Mouse

Sample Type：serum
Association of C5a/C5aR pathway to activate ERK1/2 and p38 MAPK in acute kidney injury – a mouse model

IF: 0.493

Journal： Revista Romana de Medicina de Laborator (2022)

DOI：10.2478/rrlm-2022-0001

Reactivity：Mouse

Sample Type：serum
3D printing monetite-coated Ti-6Al-4V surface with osteoimmunomodulatory function to enhance osteogenesis

Journal： Biomaterials Advances (2022)

DOI：10.1016/j.msec.2021.112562

Reactivity：Mouse
Anti-Inflammatory, ACE Inhibitory, Antioxidative Activities and Release of Novel Antihypertensive and Antioxidative Peptides from Whey Protein Hydrolysate with Molecular Interactions

Journal： Journal of the American Nutrition Association (2022)

DOI：10.1080/07315724.2022.2052201

Reactivity：Mouse
Purification and Characterization of Novel Antihypertensive and Antioxidative Peptides From Whey Protein Fermentate: In Vitro, In Silico, and Molecular Interactions Studies

Journal： Journal of the American Nutrition Association (2022)

DOI：10.1080/27697061.2022.2110966

Reactivity：Mouse

Species

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Human

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