Mini Sample Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：微量法小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-MSEL-M0002
文献（1562）
说明书

说明书

别称：

DIF、Tnfa、TNF-a、TNFSF2、Tnlg1f、Tnfsf1a、TNFalpha、TNF-alpha、Tnf、Cachectin、Tumor necrosis factor、Tumor necrosis factor ligand superfamily member 2

Mini Sample Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥3080.00 ¥2180.00 ¥13860.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 7.81-500 pg/mL
灵敏度： 4.69 pg/mL

系列介绍	MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4，能有效解决实验样本体积不够的问题。Elabscience^®自主开发的新技术，旨在帮助您以更高效的方式进行科学研究。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中TNF-α浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	7.81-500 pg/mL
灵敏度	4.69 pg/mL
样本体积	25 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤

Sweroside ameliorates IMQ-induced psoriasiform inflammation by inhibiting NLRP3/Caspase-1 mediated IL-1β elevation

IF: 4.8

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)

DOI：10.1016/j.intimp.2025.114333

Reactivity：Mouse

Sample Type：serum
Inhibiting HMGB1/AGER/NF-κB pathway prevents pro-inflammatory microglia polarization and protect photoreceptors in retinitis pigmentosa

IF: 4.8

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)

DOI：10.1016/j.intimp.2025.114192

Reactivity：Mouse
Exploring macrophage and nerve interaction in endometriosis-associated pain: the inductive role of IL-33

IF: 4.8

Journal： INFLAMMATION RESEARCH (2025)

DOI：10.1007/s00011-025-02010-x

Reactivity：Mouse

Sample Type：serum,peritoneal fluid (PF)
Fluorofenidone mitigates liver fibrosis through GSK-3β modulation and hepatocyte protection in a 3D tissue-engineered model

IF: 4.8

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)

DOI：10.1016/j.intimp.2025.114209

Reactivity：Mouse

Sample Type：liver tissue
Network pharmacology integrated with experimental validation reveals the mechanism of Xanthii Fructus against allergic rhinitis via JAK2/STAT3/HIF-1α signaling pathway

IF: 4.8

Journal： JOURNAL OF ETHNOPHARMACOLOGY (2025)

DOI：10.1016/j.jep.2025.119461

Reactivity：Mouse

Sample Type：serum
Chemical structures, antioxidant capacities, and immunostimulatory activities of pectic polysaccharides from jujube fruits collected from different producing areas

IF: 4.8

Journal： Food Bioscience (2025)

DOI：10.1016/j.fbio.2025.106485

Reactivity：Mouse
Explore the key targets and mechanism of Danggui Buxue decoction against ulcerative colitis: Network pharmacology and experimental validation

IF: 4.8

Journal： JOURNAL OF ETHNOPHARMACOLOGY (2025)

DOI：10.1016/j.jep.2025.119580

Reactivity：Mouse

Sample Type：Colon tissue
Rosa roxburghii juice alleviates DEHP-induced reproductive system damage in male mice via the PI3K/AKT signaling pathway

IF: 4.8

Journal： JOURNAL OF ETHNOPHARMACOLOGY (2025)

DOI：10.1016/j.jep.2025.119742

Reactivity：Mouse

Sample Type：Testicular tissue
Parental high-fat diet induces upregulation of macrophage receptor with collagenous structure expression and exacerbates colorectal inflammation via the nuclear factor kappa-B pathway in offspring

IF: 4.8

Journal： JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2025)

DOI：10.1016/j.jnutbio.2025.109918

Reactivity：Mouse

Sample Type：serum
TJ0113 attenuates fibrosis in metabolic dysfunction-associated steatohepatitis by inducing mitophagy

IF: 4.8

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)

DOI：10.1016/j.intimp.2025.114678

Reactivity：Mouse

Sample Type：serum

Species

{{relatedElisaPage.numTotal}} Results

All

Canine

Human

Mouse

Porcine

Rat

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual

Cat.No.：{{item.cat}}

{{index}}{{goods_show_value}}