Mini Sample Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：微量法小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-MSEL-M0002
文献（1562）
说明书

说明书

别称：

DIF、Tnfa、TNF-a、TNFSF2、Tnlg1f、Tnfsf1a、TNFalpha、TNF-alpha、Tnf、Cachectin、Tumor necrosis factor、Tumor necrosis factor ligand superfamily member 2

Mini Sample Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥3080.00 ¥2180.00 ¥13860.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 7.81-500 pg/mL
灵敏度： 4.69 pg/mL

系列介绍	MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4，能有效解决实验样本体积不够的问题。Elabscience^®自主开发的新技术，旨在帮助您以更高效的方式进行科学研究。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中TNF-α浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	7.81-500 pg/mL
灵敏度	4.69 pg/mL
样本体积	25 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤

Biomimetic Tertiary Lymphoid Structures with Microporous Annealed Particle Scaffolds for Cancer Postoperative Therapy

IF: 17.1

Journal： ACS Nano (2024)

DOI：10.1021/acsnano.4c01180

Sample Type：bone marrow-derived dendritic cells (BMDCs)
Ultrasound-Guided Drug Delivery System Utilizing Piezocatalytic MoS2 Nanomaterials for Anti-Inflammatory Therapy

IF: 16.8

Journal： Nano Energy (2024)

DOI：10.1016/j.nanoen.2024.109732

Reactivity：Mouse
Genetically Edited Cascade Nanozymes for Cancer Immunotherapy

IF: 15.8

Journal： ACS Nano (2024)

DOI：10.1021/acsnano.4c01229

Reactivity：Mouse

Sample Type：4T1 cell-Xenograft
Modulating Elasticity of Liposome for Enhanced Cancer Immunotherapy

IF: 15.8

Journal： ACS Nano (2024)

DOI：10.1021/acsnano.4c09094

Reactivity：Mouse
Engineering AIEgens-Tethered Gold Nanoparticles with Enzymatic Dual Self-Assembly for Amplified Cancer-Specific Phototheranostics

IF: 15.8

Journal： ACS Nano (2024)

DOI：10.1021/acsnano.4c07403

Reactivity：Mouse

Sample Type：Serum
Rewiring Tryptophan Metabolism via Programmable Probiotic Integrated by Dual-Layered Microcapsule Protects against Inflammatory Bowel Disease in Mice

IF: 15.8

Journal： ACS Nano (2024)

DOI：10.1021/acsnano.4c12801

Reactivity：Mouse

Sample Type：colon tissue
Milk-derived extracellular vesicles functionalized with anti-tumour necrosis factor-α nanobody and anti-microbial peptide alleviate ulcerative colitis in mice

IF: 15.5

Journal： Journal of Extracellular Vesicles (2024)

DOI：10.1002/jev2.12462

Reactivity：Mouse

Sample Type：colon tissue
A single-domain antibody targeting factor XII inhibits both thrombosis and inflammation

IF: 14.7

Journal： Nature Communications (2024)

DOI：10.1038/s41467-024-51745-4

Reactivity：Mouse

Sample Type：plasma
Dendritic nanomedicine enhances chemo-immunotherapy by disturbing metabolism of cancer-associated fibroblasts for deep penetration and activating function of immune cells

IF: 14.5

Journal： Acta Pharmaceutica Sinica B (2024)

DOI：10.1016/j.apsb.2024.03.010

Sample Type：Bone marrow-derived dendritic cells (BMDCs)
H3K4me3-Mediated FOXJ2/SLAMF8 Axis Aggravates Thrombosis and Inflammation in β2GPI/Anti-β2GPI-Treated Monocytes

IF: 14.3

Journal： Advanced Science (2024)

DOI：10.1002/advs.202309140

Reactivity：Mouse

Sample Type：serum

Species

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Canine

Human

Mouse

Porcine

Rat

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