Mini Sample Mouse IFN-γ (Interferon Gamma) ELISA Kit

中文名称：微量法小鼠γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒

货号：E-MSEL-M0007
文献（288）
说明书

说明书

别称：

Ifng

Mini Sample Mouse IFN-γ (Interferon Gamma) ELISA Kit

Price: ¥3080.00 ¥2180.00 ¥13860.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 7.81-500 pg/mL
灵敏度： 4.53 pg/mL

系列介绍	MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4，能有效解决实验样本体积不够的问题。Elabscience^®自主开发的新技术，旨在帮助您以更高效的方式进行科学研究。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IFN-γ浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IFN-γ会与包被抗体结合。后依次加入生物素化的抗小鼠IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗小鼠IFN-γ抗体与结合在包被抗体上的小鼠IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	7.81-500 pg/mL
灵敏度	4.53 pg/mL
样本体积	25 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠IFN-γ，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤

Alcohol intake exacerbates experimental autoimmune prostatitis through activating PI3K/AKT/mTOR pathway-mediated Th1 differentiation

IF: 5.7

Journal： Frontiers in Immunology (2025)

DOI：10.3389/fimmu.2024.1512456

Reactivity：Mouse

Sample Type：plasma
A syringeable immunotherapeutic hydrogel enhances T cell immunity via in-situ activation of STING pathway for advanced breast cancer postoperative therapy

IF: 5.7

Journal： Frontiers in Immunology (2025)

DOI：10.3389/fimmu.2025.1523436

Reactivity：Mouse

Sample Type：Serum,E0771 cell-Xenograft
Miquelianin inhibits IAV infection via the MAPK signaling pathway both in vitro and in vivo

IF: 5.7

Journal： Frontiers in Immunology (2025)

DOI：10.3389/fimmu.2025.1532336

Reactivity：Mouse

Sample Type：serum
Exploring the role of LOX family in glioma progression and immune modulation

IF: 5.7

Journal： Frontiers in Immunology (2025)

DOI：10.3389/fimmu.2025.1512186

Reactivity：Mouse

Sample Type：GL261 cell-Xenograft
Curcumin exerts anti-tumor activity in colorectal cancer via gut microbiota-mediated CD8+ T Cell tumor infiltration and ferroptosis

IF: 5.1

Journal： Food & Function (2025)

DOI：10.1039/D4FO04045G

Reactivity：Mouse

Sample Type：serum
Endogenous ERMAP Affects T-Cell Function in EAE Mice

IF: 4.9

Journal： IMMUNOLOGY (2025)

DOI：10.1111/imm.13910

Reactivity：Mouse

Sample Type：serum
Development and Characterization of a Recombinant galT-galU Protein for Broad-Spectrum Immunoprotection Against Porcine Contagious Pleuropneumonia

IF: 4.9

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2025)

DOI：10.3390/ijms26083634

Reactivity：Mouse

Sample Type：serum
The Evaluation of the Anti-inflammatory effects of Acorus calamus L. Ethanolic extract on Ovalbumin-induced allergic Asthma in Mice

IF: 4.8

Journal： JOURNAL OF ETHNOPHARMACOLOGY (2025)

DOI：10.1016/j.jep.2025.119903

Reactivity：Mouse

Sample Type：Bronchoalveolar lavage fluid (BALF)
Epimedii Folium and Curculiginis Rhizoma ameliorate age-related cognitive decline and neuroinflammation through modulation of the NLRP3 inflammasome

IF: 4.8

Journal： JOURNAL OF ETHNOPHARMACOLOGY (2025)

DOI：10.1016/j.jep.2025.119883

Reactivity：Mouse

Sample Type：serum
Strong immune response and protection against Brucella abortus by Omp25 and BP26 mRNA vaccine candidates

IF: 4.8

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)

DOI：10.1016/j.intimp.2025.114765

Reactivity：Mouse

Sample Type：serum

Species

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Canine

Human

Mouse

Porcine

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