Mini Sample Mouse IgM (Immunoglobulin M) ELISA Kit
中文名称：微量法小鼠免疫球蛋白M(IgM)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Mouse
- 检测范围： 7.81-500 ng/mL
- 灵敏度： 4.21 ng/mL
| 系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4，能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术，旨在帮助您以更高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IgM浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IgM抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IgM会与包被抗体结合。后依次加入生物素化的抗小鼠IgM抗体和辣根过氧化物酶标记的亲和素，抗小鼠IgM抗体与结合在包被抗体上的小鼠IgM结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IgM浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IgM的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 ng/mL |
| 灵敏度 | 4.21 ng/mL |
| 样本体积 | 25 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IgM，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
IQGAP1 promotes early B cell development, is essential for the development of marginal zone (MZ) B cells, and is critical for both T-dependent and T-independent antibody responses
IF: 6.200Journal： CELLULAR AND MOLECULAR LIFE SCIENCES (2024)Reactivity：MouseSample Type：seraExopolysaccharides and Surface-Layer Proteins Expressed by Biofilm-State Lactiplantibacillus plantarum Y42 Play Crucial Role in Preventing Intestinal Barrier and Immunity Dysfunction of Balb/C Mice Infected by Listeria monocytogenes ATCC 19115
IF: 5.700Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2024)Reactivity：MouseSample Type：serumEvaluation of the immunomodulatory effects of a novel synbiotic made of combined use of probiotic-prebiotic-Chinese traditional herbs
IF: 4.800Journal： Journal of Agriculture and Food Research (2024)Reactivity：MouseSample Type：serumSingle-dose of hybrid eukaryotic-prokaryotic nanovesicles to drive a rapid and controllable immune response against Klebsiella pneumoniae induced nosocomial pneumonia
IF: 17.400Journal： Nano Today (2023)Reactivity：MouseSample Type：broncho alveolar lavage fluid (BALF)Immuno-stimulatory activity of Astragalus polysaccharides in cyclophosphamide-induced immunosuppressed mice by regulating gut microbiota
IF: 8.200Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2023)PMID：37164141Reactivity：MouseSample Type：plasmaLactobacillus paracasei influences the gut-microbiota-targeted metabolic modulation of the immune status of diarrheal mice
IF: 6.100EZH2 inhibition dampens autoantibody production in lupus by restoring B cell immune tolerance
IF: 5.600Tripterygium hypoglaucum extract ameliorates adjuvant-induced arthritis in mice through the gut microbiota
IF: 4.600Journal： Chinese Journal of Natural Medicines (2023)PMID：37879792Reactivity：MouseSample Type：plasmaStructural characterization and immunomodulatory effect of a starch-like Grifola frondosa polysaccharides on cyclophosphamide-induced immunosuppression in mice
IF: 3.100cGAS and DDX41-STING mediated intrinsic immunity spreads intercellularly to promote neuroinflammation in SOD1 ALS model
IF: 6.107
