Mini Sample Mouse IL-10 (Interleukin 10) ELISA Kit
中文名称：微量法小鼠白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.13 pg/mL
| 系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4，能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术，旨在帮助您以更高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-10浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-10抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-10会与包被抗体结合。后依次加入生物素化的抗小鼠IL-10抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-10抗体与结合在包被抗体上的小鼠IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.13 pg/mL |
| 样本体积 | 25 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
-
Hydrolysis of 2D Nanosheets Reverses Rheumatoid Arthritis Through Anti-Inflammation and Osteogenesis
IF: 27.400 -
A Dual Enhancing Strategy of Novel Nanovaccine Based on TIM3 Silencing Nanoadjuvants and Desialylated Cancer Cell Membrane Antigens for Personalized Vaccination Immunotherapy of Cancer
IF: 18.500Journal： ADVANCED FUNCTIONAL MATERIALS (2024)Reactivity：MouseSample Type：lymph node-derived single-cell suspension -
An Adjuvant Micelle-Based Multifunctional Nanosystem for Tumor Immunotherapy by Remodeling Three Types of Immunosuppressive Cells
IF: 17.100Journal： ACS Nano (2024) -
Milk-derived extracellular vesicles functionalized with anti-tumour necrosis factor-α nanobody and anti-microbial peptide alleviate ulcerative colitis in mice
IF: 15.500Journal： Journal of Extracellular Vesicles (2024)Reactivity：MouseSample Type：colon tissue -
Spatiotemporally Controlled T-Cell Combination Therapy for Solid Tumor
IF: 14.300 -
Macrophage-derived biomimetic nanoparticles enhanced SDT combined with immunotherapy inhibited tumor growth and metastasis
IF: 14.000Journal： BIOMATERIALS (2024)Sample Type：RAW 264.7 macrophages -
Rational design of ICD-inducing nanoparticles for cancer immunotherapy
IF: 13.600Journal： Science Advances (2024) -
Dual-Drug nanoparticles activate pyroptosis in cancer cells synergistically target cancer stem cells to augment chemoimmunotherapy of triple-negative breast cancer
IF: 13.300Journal： CHEMICAL ENGINEERING JOURNAL (2024)Reactivity：MouseSample Type：serum -
Self-assembled herbal hydrogel for rectal administration therapy in ulcerative colitis
IF: 13.300 -
Natural Polyphenol Delivered Methylprednisolone Achieve Targeted Enrichment for Acute Spinal Cord Injury Therapy
IF: 13.000
实验操作视频
