Mini Sample Mouse IL-12 (Interleukin 12) ELISA Kit

中文名称：微量法小鼠白介素12(IL-12)酶联免疫吸附测定试剂盒

货号：E-MSEL-M0004
文献（71）
说明书

说明书

别称：

CLMF、CLMF p、CLMF p40、CLMF2、Cytotoxic lymphocyte maturation factor 40 kDa subunit、Cytotoxic Lymphocyte Maturation Factor 40 kDa subunit (CLMF p40)、IL 12B、IL12、IL12 p、IL-12 p、IL-12 p40、IL12 p70、IL-12 p70、IL-12 subunit p、IL-12 subunit p40、IL-12/IL-23 p、IL-12/IL-23 p40、Il12b、IL-12B、

Mini Sample Mouse IL-12 (Interleukin 12) ELISA Kit

Price: ¥3080.00 ¥2180.00 ¥13860.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 0.31-20 pg/mL
灵敏度： 0.19 pg/mL

系列介绍	MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4，能有效解决实验样本体积不够的问题。Elabscience^®自主开发的新技术，旨在帮助您以更高效的方式进行科学研究。
用途	试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-12浓度
产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-12抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-12会与包被抗体结合。后依次加入生物素化的抗小鼠IL-12抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-12抗体与结合在包被抗体上的小鼠IL-12结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-12浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-12的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	0.31-20 pg/mL
灵敏度	0.19 pg/mL
样本体积	25 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠IL-12，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤

An Adjuvant Micelle-Based Multifunctional Nanosystem for Tumor Immunotherapy by Remodeling Three Types of Immunosuppressive Cells

IF: 17.100

Journal： ACS Nano (2024)

DOI：10.1021/acsnano.3c08792
Ruthenium(II) Lipid-Mimics Drive Lipid Phase Separation to Arouse Autophagy-Ferroptosis Cascade for Photoimmunotherapy

IF: 14.300

Journal： Advanced Science (2024)

DOI：10.1002/advs.202411629

Reactivity：Mouse

Sample Type：Serum
Ultrasound-Activated Precise Sono-Immunotherapy for Breast Cancer with Reduced Pulmonary Fibrosis

IF: 14.300

Journal： Advanced Science (2024)

DOI：10.1002/advs.202407609

Reactivity：Mouse

Sample Type：4T1 cell-Xenograft
Dual-Drug nanoparticles activate pyroptosis in cancer cells synergistically target cancer stem cells to augment chemoimmunotherapy of triple-negative breast cancer

IF: 13.300

Journal： CHEMICAL ENGINEERING JOURNAL (2024)

DOI：10.1016/j.cej.2024.158944

Reactivity：Mouse

Sample Type：serum
Single-cell data-driven design of armed oncolytic virus and combination therapy to activate a cooperative innate-adaptive immunity against cancer

IF: 12.100

Journal： MOLECULAR THERAPY (2024)

DOI：10.1016/j.ymthe.2024.12.017

Reactivity：Mouse

Sample Type：Pan02 cell-Xenograft
Site-specific controlled-release nanoparticles for immune reprogramming via dual metabolic inhibition against triple-negative breast cancer

IF: 10.800

Journal： JOURNAL OF CONTROLLED RELEASE (2024)

DOI：10.1016/j.jconrel.2023.12.022
Tumor Microenvironment Reprogrammed Bimetallic Hybrid Nanostimulator for Triggering Radio-Cuproptosis-Immunotherapy

IF: 10.000

Journal： Advanced Healthcare Materials (2024)

DOI：10.1002/adhm.202401902

Reactivity：Mouse

Sample Type：serum
Tumor Microenvironment-Responsive Macrophage-Mediated Immunotherapeutic Drug Delivery

IF: 9.400

Journal： Acta Biomaterialia (2024)

DOI：10.1016/j.actbio.2024.07.042

Reactivity：Mouse

Sample Type：serum
Metabolic regulating enhanced immunological activating nanocomposites for tumor microenvironment normalization and immunotherapy

IF: 8.200

Journal： Materials Today Nano (2024)

DOI：10.1016/j.mtnano.2024.100509

Reactivity：Mouse

Sample Type：serum
β-Hydroxybutyrate suppresses M1 macrophage polarization through β-hydroxybutyrylation of the STAT1 protein

IF: 8.100

Journal： Cell Death & Disease (2024)

DOI：10.1038/s41419-024-07268-3

Reactivity：Mouse

Species

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Mouse

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