Mini Sample Mouse KIM-1 (Kidney Injury Molecule 1) ELISA Kit
中文名称：微量法小鼠肾损伤分子1(KIM-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4，能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术，旨在帮助您以更高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中KIM-1浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠KIM-1抗体包被于酶标板上，实验时样品（或标准品）中的小鼠KIM-1会与包被抗体结合。后依次加入生物素化的抗小鼠KIM-1抗体和辣根过氧化物酶标记的亲和素，抗小鼠KIM-1抗体与结合在包被抗体上的小鼠KIM-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，KIM-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中KIM-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 25 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠KIM-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
-
Hierarchical Targeting Nanodrug with Holistic DNA Protection for Effective Treatment of Acute Kidney Injury
IF: 14.300Journal： Advanced Science (2024)Reactivity：MouseSample Type：kidney tissue -
Deficiency of thiosulfate sulfurtransferase mediates the dysfunction of renal tubular mitochondrial fatty acid oxidation in diabetic kidney disease
IF: 13.700Journal： CELL DEATH AND DIFFERENTIATION (2024)Reactivity：MouseSample Type：urine -
Circadian light/dark cycle reversal exacerbates the progression of chronic kidney disease in mice
IF: 8.300 -
Dynamic changes in the real-time glomerular filtration rate and kidney injury markers in different acute kidney injury models
IF: 6.100Journal： Journal of Translational Medicine (2024)Reactivity：MouseSample Type：kidney tissue -
Dipeptidase 1 promotes ferroptosis in renal tubular epithelial cells in diabetic nephropathy via inhibition of the GSH/GPX4 axis
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：urine -
Unveiling the role of mtDNA in Liver-Kidney Crosstalk: Insights from trichloroethylene hypersensitivity syndrome
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：Serum -
Indole-3-carboxaldehyde alleviates cisplatin-induced acute kidney injury in mice by improving mitochondrial dysfunction via PKA activation
IF: 4.300Journal： FOOD AND CHEMICAL TOXICOLOGY (2024) -
Peficitinib halts acute kidney injury via JAK/STAT3 and growth factors immunomodulation
IF: 4.200Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)Reactivity：MouseSample Type：kidney tissue -
Nrf2/FSP1/CoQ10 axis-mediated ferroptosis is involved in sodium aescinate-induced nephrotoxicity
IF: 3.800Journal： ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2024)Reactivity：MouseSample Type：serum -
Protective effect and mechanism of CoQ10 in mitochondrial dysfunction in diquat-induced renal proximal tubular injury
IF: 3.200Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2024)Reactivity：MouseSample Type：Serum
实验操作视频
