Mini Sample Mouse SELE (E-selectin) ELISA Kit
中文名称:微量法小鼠E选择素(SELE)酶联免疫吸附测定试剂盒

Price:
- 反应性: Mouse
- 检测范围: 0.31-20 ng/mL
- 灵敏度: 0.19 ng/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中SELE浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠SELE抗体包被于酶标板上,实验时样品(或标准品)中的小鼠SELE会与包被抗体结合。后依次加入生物素化的抗小鼠SELE抗体和辣根过氧化物酶标记的亲和素,抗小鼠SELE抗体与结合在包被抗体上的小鼠SELE结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,SELE浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中SELE的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Mouse |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.31-20 ng/mL |
灵敏度 | 0.19 ng/mL |
样本体积 | 25 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠SELE,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
-
Wireless Optogenetic Targeting Nociceptors Helps Host Cells Win the Competitive Colonization in Implant-Associated Infections
IF: 10.700Journal: Small Methods (2024)Reactivity:MouseSample Type:proximal tibial tissue,surrounding tissue -
Nattokinase attenuates endothelial inflammation through the activation of SRF and THBS1
IF: 7.700Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)Reactivity:MouseSample Type:serum -
Investigation of the efficacy of Dengzhan Shengmai capsule against heart failure with preserved ejection fraction
IF: 4.800Journal: JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity:MouseSample Type:serum -
Chronic exposure to UVB induces nephritis and gut microbiota dysbiosis in mice based on the integration of renal transcriptome profiles and 16S rRNA sequencing data
IF: 8.900 -
Retinol-Binding Protein 4 Accelerates Metastatic Spread and Increases Impairment of Blood Flow in Mouse Mammary Gland Tumors
IF: 6.126 -
Temporal inhibition of mouse mammary gland cancer metastasis by CORM-A1 and DETA/NO combination therapy
IF: 8.063 -
Oral administration of 3,3′-diselenodipropionic acid prevents thoracic radiation induced pneumonitis in mice by suppressing NF-kB/IL-17/G-CSF/neutrophil axis
IF: 5.657Journal: FREE RADICAL BIOLOGY AND MEDICINE (2019)PMID:31521664Reactivity:MouseSample Type:Bronchoalveolar Lavage Fluid,Tissue homogenate