Mouse HMGB-1(Highmobility group protein B1) ELISA Kit
中文名称：小鼠高迁移率族蛋白B1(HMGB-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠HMGB-1抗体包被于酶标板上，实验时样品（或标准品）中的小鼠HMGB-1会与包被抗体结合。后依次加入生物素化的抗小鼠HMGB-1抗体和辣根过氧化物酶标记的亲和素，抗小鼠HMGB-1抗体与结合在包被抗体上的小鼠HMGB-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，HMGB-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中HMGB-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠HMGB-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Vanadyl nanocomplexes enhance photothermia-induced cancer immunotherapy to inhibit tumor metastasis and recurrence
IF: 12.479 -
Polymeric PD-L1 blockade nanoparticles for cancer photothermal-immunotherapy
IF: 12.479 -
Enhancing cancer chemo-immunotherapy by biomimetic nanogel with tumor targeting capacity and rapid drug-releasing in tumor microenvironment
IF: 11.614 -
Systemic administration of polymersomal oncolytic peptide LTX-315 combining with CpG adjuvant and anti-PD-1 antibody boosts immunotherapy of melanoma
IF: 9.776 -
Laser/GSH-Activatable Oxaliplatin/Phthalocyanine-Based Coordination Polymer Nanoparticles Combining Chemophotodynamic Therapy to Improve Cancer Immunotherapy
IF: 9.229 -
Immunogenic Cell Death by the Novel Topoisomerase I Inhibitor TLC388 Enhances the Therapeutic Efficacy of Radiotherapy
IF: 6.639 -
Liposomal remdesivir inhalation solution for targeted lung delivery as a novel therapeutic approach for COVID-19
IF: 6.598Journal： Asian Journal of Pharmaceutical Sciences (2021)PMID：34703490Reactivity：MouseSample Type：serum BALF -
The receptor for advanced glycation end products mediates dysfunction of airway epithelial barrier in a lipopolysaccharides-induced murine acute lung injury model
IF: 4.932Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2021)PMID：33548580Reactivity：MouseSample Type：Bronchoalveolar Lavage Fluid -
Osthole Blocks HMGB1 Release From the Nucleus and Confers Protective Effects Against Renal Ischemia-Reperfusion Injury
IF: 4.566 -
Hydrogen Alleviates Neuronal Injury and Neuroinflammation Induced by Microglial Activation via the Nuclear Factor Erythroid 2-related Factor 2 Pathway in Sepsis-associated Encephalopathy
IF: 3.590Journal： NEUROSCIENCE (2021)PMID：33992722Reactivity：Rat,MouseSample Type：Cell culture supernatant,Tissue homogenate(Brain)
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
