Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
中文名称：小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
AM1241 inhibits chondrocyte inflammation and ECM degradation through the Nrf2/HO-1 and NF-κB pathways and alleviates osteoarthritis in mice
IF: 6.0 -
Instantaneous dose rate as a crucial factor in reducing mortality and normal tissue toxicities in murine total-body irradiation: a comparative study of dose rate combinations
IF: 6.0Journal： MOLECULAR MEDICINE (2025)Reactivity：MouseSample Type：Serum -
S-propargyl-cysteine attenuates temporomandibular joint osteoarthritis by regulating macrophage polarization via Inhibition of JAK/STAT signaling
IF: 6 -
Luteolin 7-Glucuronide in Artemisia rupestris L. Extract Attenuates Pulmonary Fibrosis by Inhibiting Fibroblast Activation and FMT via Targeting of TGF-β1
IF: 6 -
Physicochemical characteristics and therapeutic mechanisms of Sargassum horneri-derived soluble dietary fiber in cyclophosphamide-induced intestinal damage via gut microbiota and metabolic modulation
IF: 5.9Journal： Food Bioscience (2025)Reactivity：MouseSample Type：Colonic tissue -
ER stress genes (COL1A1, LOXL2, VWF) predicts IKK-16 as a Candidate therapeutic target for colitis-related inflammation and fibrosis suppression
IF: 5.9Journal： Frontiers in Immunology (2025)Reactivity：MouseSample Type：serum -
Alcohol intake exacerbates experimental autoimmune prostatitis through activating PI3K/AKT/mTOR pathway-mediated Th1 differentiation
IF: 5.7Journal： Frontiers in Immunology (2025)Reactivity：MouseSample Type：plasma -
A syringeable immunotherapeutic hydrogel enhances T cell immunity via in-situ activation of STING pathway for advanced breast cancer postoperative therapy
IF: 5.7Journal： Frontiers in Immunology (2025)Reactivity：MouseSample Type：Serum,E0771 cell-Xenograft -
Hypoglycemic and Gut Microbiota-Modulating Effects of Pectin from Citrus aurantium “Changshanhuyou” Residue in Type 2 Diabetes Mellitus Mice
IF: 5.7Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2025)Reactivity：MouseSample Type：serum -
Flavor Profile of 4-Isothiocyanato-1-butene in Microwave Rapeseed Oil and Its Anti-Inflammatory Properties In Vitro
IF: 5.7Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2025)Reactivity：Mouse
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
